Sister chromatid exchange rate and alkaline comet assay scores in patients with ovarian cancer
Sister chromatid exchange rate and alkaline comet assay scores in patients with ovarian cancer
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DOI：
10.1006/gyno.2001.6450
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发表时间：
2002-01-01
影响因子：
4.7
通讯作者：
Haberal, A
中科院分区：
文献类型：
--
作者：
Baltaci, V;Kayikçioglu, F;Haberal, A
客观的。研究人员对不同类型卵巢恶性肿瘤患者和健康志愿者的姐妹染色单体交换（SCE）频率进行了研究。还通过碱性单细胞凝胶电泳 (SCGE)（也称为彗星测定）研究了卵巢恶性肿瘤患者和对照受试者的 DNA 损伤水平。患者和方法。采集组织学证实为恶性肿瘤的 30 名患者和 20 名健康女性志愿者的外周血。根据细胞的损伤程度对细胞进行评估。结果。我们发现癌症病例的姐妹染色单体交换频率显着高于对照组（P < 0.001）。两组染色体组 A、B 和 C（包括 1-12 号染色体）的交换频率均高于其他染色体组。患者和对照受试者的碱性彗星试验结果比较显示，受损细胞的数量存在显着差异。卵巢恶性肿瘤女性中有限迁移细胞和广泛迁移细胞的频率均高于对照女性(P<0.001)。结论。 SCE 和 SCGE 可成功用于监测卵巢癌女性的 DNA 损伤。 (C) 2001 年爱思唯尔科学。
Objective. Sister chromatid exchange (SCE) frequencies were studied in patients with different types of ovarian malignancies and in healthy volunteers. The level of DNA damage in patients with ovarian malignancy and control subjects has also been studied by alkaline single cell gel electrophoresis (SCGE), also known as the comet assay.Patients and methods. Peripheral blood was collected from 30 patients after histological confirmation of malignancy and 20 healthy female volunteers. The cells were evaluated according to their grade of damage.Results. We found that the sister chromatid exchange frequencies of cancer cases were significantly greater than that of controls (P < 0.001). The frequency of exchange in chromosomal groups A, B, and C, which include chromosomes 1-12, was higher than that of the other chromosomal groups in both groups. Comparison of the results of the alkaline comet assay in patient and control subjects showed a significant difference in the number of damaged cells. The frequency of limited migrated and extensive migrated cells in the women with ovarian malignancies was higher than that of control women (P < 0.001).Conclusion. SCE and SCGE can be used successfully to monitor DNA damage in women with ovarian cancer. (C) 2001 Elsevier Science.