CrAssphage abundance and correlation with molecular viral markers in Italian wastewater
CrAssphage abundance and correlation with molecular viral markers in Italian wastewater
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DOI：
10.1016/j.watres.2020.116161
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发表时间：
2020-10-01
期刊：
影响因子：
12.8
通讯作者：
Bibby, Kyle
中科院分区：
文献类型：
--
作者：
Crank, Katherine;Li, Xiang;Bibby, Kyle
目前用于环境健康监测的粪便指示物主要基于粪便指示细菌（FIB），其不能准确地代表病毒病原体。需要高度丰富的人类相关病毒粪便指示物来代表污水污染水中的病毒病原体。在本研究中，我们评估了2014年至2018年期间收集的156个意大利废水样本中新出现的病毒粪便指示剂crAsperage的丰度。使用两种单独的病毒浓缩方法（甘氨酸-CF和PEG-葡聚糖）收集样品，并对crAsA病毒（CPQ 56）和人多瘤病毒（HPyV）进行qPCR测定，对人博卡病毒（HBoc）和戊型肝炎病毒（HepE）进行终点PCR测定。在96%的样品中检测到CrAs年龄，并且在浓缩方法之间未观察到CrAs年龄丰度的统计学显著差异（p 1/4 0.39）。铬砷浓度也没有相关的位置（纬度）或大小（负荷和容量）的污水处理厂。甘氨酸-CF法和PEG-葡聚糖法的HPyV检出率分别为64%和100%，浓度法对HPyV浓度的影响具有统计学意义（p < 0.0001）。对于两种浓度方法，在显著高于HPyV的浓度下测量CrAsA年龄（p < 0.0001）。测得的crAsA和HPyV的浓度范围分别为3.84-7.29 log（10）GC/100 mL和3.45-5.17 log（10）GC/100 mL。有一个很强的正相关性crAsbriage和HPyV丰度的两种浓度的方法，但是，相关性的斜率依赖于浓度的方法。CrAspherage的存在与甘氨酸-CF浓缩样品中HBoc的存在相关，但与PEG-葡聚糖法浓缩的HBoc的存在或HepE的存在无关。总体而言，这些结果表明，crAsA是废水中丰富的病毒粪便指示物，与人类病毒病原体具有统计学显著相关性（例如，HPyV）和病毒浓度方法影响粪便病毒指示物检测的解释。(c)2020爱思唯尔有限公司保留所有权利。
Current fecal indicators for environmental health monitoring are primarily based on fecal indicator bacteria (FIB) which do not accurately represent viral pathogens. There is a need for highly abundant, human-associated viral fecal indicators to represent viral pathogens in sewage-contaminated water. In the present study, we evaluate the abundance of the emerging viral fecal indicator crAssphage in 156 Italian wastewater samples collected between 2014 and 2018. Samples were collected using two separate viral concentration methods, glycine-CF and PEG-dextran and qPCR assays were run for crAssphage (CPQ56) and Human Polyomavirus (HPyV) and endpoint PCR assays were run for Human Bocavirus (HBoc) and Hepatitis E Virus (HepE). CrAssphage was detected in 96% of samples and no statistically significant difference was observed in crAssphage abundance between concentration methods (p 1/4 0.39). CrAssphage concentrations also did not correlate with location (latitude) or size (load and capacity) of the wastewater treatment plant. HPyV detection rates with the glycine-CF and PEG-dextran methods were 64% and 100%, respectively, and the concentrations of HPyV were statistically significantly influenced by the concentration method (p < 0.0001). CrAssphage was measured at significantly higher concentrations than HPyV for both concentration methods (p < 0.0001). The observed concentration ranges were 3.84-7.29 log(10)GC/10 0 mL for crAssphage and 3.45-5.17 log(10)GC/10 0 mL for HPyV. There was a strong positive correlation between crAssphage and HPyV abundance for both concentration methods; however, the slope of the correlation depended on the concentration method. CrAssphage presence correlated with the presence of HBoc in samples concentrated with glycine-CF, but did not correlate with the presence of HBoc concentrated with the PEG-dextran method or with the presence of HepE. Overall, these results demonstrate that crAssphage is an abundant viral fecal indicator in waste-water with statistically significant correlation with human viral pathogens (e.g., HPyV) and viral concentration methods influence the interpretation of fecal viral indicator detection. (c) 2020 Elsevier Ltd. All rights reserved.