Novel approach to controlled protein crystallization through ligandation of yttrium cations
Novel approach to controlled protein crystallization through ligandation of yttrium cations
复制标题
通过钇阳离子配体控制蛋白质结晶的新方法
DOI：
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
F. Schreiber
中科院分区：
文献类型：
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作者：
Fajun Zhang;G. Zocher;A. Sauter;T. Stehle;F. Schreiber
由于缺乏适合于衍射实验的晶体，大分子的晶体结构测定常常受到阻碍。本文描述了一种在Y存在下使酸性蛋白质牛β-乳球蛋白结晶的方案，以产生属于新空间群的高质量晶体。Y离子不仅用于工程结晶，而且是晶格不可分割的一部分，因此可以用反常色散方法来解决相问题。首先利用蛋白质和盐浓度平面上的实验相图优化了蛋白质结晶条件。晶体生长强烈依赖于相图中的位置，最好的晶体生长在相变边界附近。结构分析表明，Y离子与表面暴露的谷氨酸和天冬氨酸侧链的结合是由晶格中不同的分子贡献的。通过以这种方式桥联分子，分子之间形成接触，从而能够形成稳定的晶格。基于其他酸性蛋白质相行为的普遍特征和多价离子诱导的相互作用，讨论了这一策略在这些蛋白质结晶中的潜在应用。
Crystal structure determination of macromolecules is often hampered by the lack of crystals suitable for diffraction experiments. This article describes a protocol to crystallize the acidic protein bovine β-lactoglobulin in the presence of yttrium to yield high-quality crystals that belong to a new space group. The yttrium ions not only are used to engineer the crystallization, but are an integral part of the crystal lattice and can therefore be used to solve the phase problem using anomalous dispersion methods. Protein crystallization conditions were first optimized using an experimental phase diagram in the protein and salt concentration plane. Crystal growth strongly depends on the position in the phase diagram, and the best crystals grow near the phase transition boundaries. The structure analysis demonstrates the specific binding of yttrium ions to surface-exposed glutamate and aspartate side chains contributed by different molecules in the crystal lattice. By bridging molecules in this manner, contacts between molecules are formed that enable the formation of a stable crystal lattice. The potential application of this strategy to the crystallization of other acidic proteins is discussed on the basis of the universal features of the phase behavior of these proteins and the interactions induced by multivalent ions.
影响因子：
4.2
作者：
P. Adams;K. Gopal;R. Grosse-Kunstleve;L. Hung;T. Ioerger;A. Mccoy;N. Moriarty;Reetal Pai;
通讯作者：
P. Adams;K. Gopal;R. Grosse-Kunstleve;L. Hung;T. Ioerger;A. Mccoy;N. Moriarty;Reetal Pai;
在没有配体的情况下，ErbB4 的细胞外区域采用系留构象
DOI：
10.1073/pnas.0507591102
发表时间：
2005-10-18
影响因子：
11.1
作者：
Bouyain, S;Longo, PA;Leahy, DJ
通讯作者：
Leahy, DJ