A ratiometric fluorescent viscosity sensor
A ratiometric fluorescent viscosity sensor
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DOI：
10.1021/ja056370a
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发表时间：
2006-01-18
影响因子：
15
通讯作者：
Theodorakis, EA
中科院分区：
文献类型：
--
作者：
Haidekker, MA;Brady, TP;Theodorakis, EA
描述了一种提供流体粘度比率测量的双探头的发展。该设计基于具有粘度无关荧光发射（蓝色单位）的初级荧光团与具有粘度敏感荧光发射量子产率（红色单位）的次级荧光团的耦合。二次荧光团的激发可以通过共振能量转移来实现。这些荧光团的荧光发射比例提供了溶剂粘度的精确比例测量。
The development of a dual probe that provides ratiometric measurements of fluid viscosity is described. The design is based on coupling of a primary fluorophore with viscosity-independent fluorescence emission (blue unit) with a secondary fluorophore that exhibits viscosity-sensitive fluorescent emission quantum yield (red unit). Excitation of the secondary fluorophore can be achieved via Resonance Energy Transfer. The ratio of the fluorescence emission of these fluorophores provides an accurate, ratiometric measurement of solvent viscosity.