Comparative in vitro study on cytotoxicity, antimicrobial activity, and binding capacity for pathophysiological factors in chronic wounds of alginate and silver-containing alginate
Comparative in vitro study on cytotoxicity, antimicrobial activity, and binding capacity for pathophysiological factors in chronic wounds of alginate and silver-containing alginate
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DOI：
10.1111/j.1524-475x.2009.00503.x
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发表时间：
2009-07-01
影响因子：
2.9
通讯作者：
Hipler, Uta-Christina
中科院分区：
文献类型：
--
作者：
Wiegand, Cornelia;Heinze, Thomas;Hipler, Uta-Christina
慢性伤口含有升高水平的蛋白酶、促炎细胞因子和自由基。细菌的存在进一步加剧了组织损伤过程。为了成功治疗，伤口敷料需要管理伤口渗出物，创造潮湿的环境，抑制感染，结合不利于伤口愈合的病理生理因素，并提供热隔离。此外，它必须减轻疼痛，易于使用，不显示过敏效力，并且不释放有毒残留物。本研究提出了一种全面的体外方法，可以评估伤口敷料，以支持伤口愈合的最佳条件。测试了三种基于藻酸盐的伤口敷料：单独的藻酸盐、含离子银的藻酸盐和含纳米晶银的藻酸盐的生物相容性、抗微生物活性以及对慢性伤口参数（如弹性蛋白酶、基质金属蛋白酶-2、肿瘤坏死因子-α、白细胞介素-8和自由基形成）的影响。发现藻酸盐结合相当数量的弹性蛋白酶，降低促炎细胞因子的浓度并抑制自由基的形成。海藻酸钠具有抗菌活性和良好的生物相容性。将银掺入藻酸盐纤维中增加了抗微生物活性，并改善了对弹性蛋白酶、基质金属蛋白酶-2和所测试的促炎细胞因子的结合亲和力。添加银也增强了抗氧化能力。然而，在体外观察到含银藻酸盐对人HaCaT角质形成细胞的明显负面影响。
Chronic wounds contain elevated levels of proteases, proinflammatory cytokines, and free radicals. The presence of bacteria further exaggerates the tissue-damaging processes. For successful treatment, the wound dressing needs to manage wound exudates, create a moist environment, inhibit infection, bind pathophysiological factors that are detrimental to wound healing, and provide thermal isolation. Furthermore, it has to relieve pain, be easy to use, show no allergic potency, and not release toxic residues. The present study suggests a comprehensive in vitro approach to enable the assessment of wound dressings to support optimal conditions for wound healing. Three alginate-based wound dressings: alginate alone, alginate containing ionic silver, and alginate with nanocrystalline silver, were tested for biocompatibility, antimicrobial activity, and influence on chronic wound parameters such as elastase, matrix metalloproteases-2, tumor necrosis factor-alpha, interleukin-8, and free radical formation. Alginate was found to bind considerable amounts of elastase, reduce the concentration of proinflammatory cytokines and inhibit the formation of free radicals. Furthermore, alginate showed antibacterial activity and high biocompatibility. Incorporation of silver into alginate fibers increased antimicrobial activity and improved the binding affinity for elastase, matrix metalloproteases-2, and the proinflammatory cytokines tested. Addition of silver also enhanced the antioxidant capacity. However, a distinct negative effect of silver-containing alginates on human HaCaT keratinocytes was noted in vitro.