Oxiradical-dependent photoemission induced by a phacoemulsification probe.
Oxiradical-dependent photoemission induced by a phacoemulsification probe.
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DOI：
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发表时间：
1992-09
影响因子：
4.4
通讯作者：
S. Shimmura;K. Tsubota;Y. Oguchi;Dai Fukumura;Makoto Suematsu;Masaharu Tsuchiya
中科院分区：
文献类型：
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作者：
S. Shimmura;K. Tsubota;Y. Oguchi;Dai Fukumura;Makoto Suematsu;Masaharu Tsuchiya
传统超声乳化术中氧自由基的形成被认为是手术中角膜内皮损伤的可能机制之一。为了验证这一假设，使用单光子计数相机和O(2)敏感的荧光素衍生物2-methyl-6-[p-methoxyphenyl-3，7-dihydroimidazo[1，2-a]吡嗪-3-酮(MCLA)可视化了超声乳化探针诱导的自由基产生，这允许可视化自由基产生的空间和时间变化。在开始超声发射后1min内，MCLA依赖的化学发光显著增强，其强度在探头顶端达到最大，并沿梯度向远端逐渐减弱。加入超氧化物歧化酶(300U/ml)或叠氮钠(20 mm o l/L)均能显著抑制细胞的化学发光。在介质中加入氘后，MCLA依赖的化学发光显著增强，表明单线态氧参与了该反应。
Oxygen free radical formation by conventional phacoemulsification devices has been postulated as a possible mechanism of corneal endothelial damage during surgery. To test this hypothesis, phacoemulsification probe-induced free radical production was visualized using a single photon-counting camera and an O(2-)-sensitive luciferin derivative, 2-methyl-6-[p-methoxyphenyl-3,7-dihydroimidazo [1,2-a]pyrazin-3-one (MCLA), which allows the visualization of spatial and temporal alterations in free radical production. Within 1 min after starting ultrasound emission, MCLA-dependent chemiluminescence was increased significantly, the intensity of which was maximal at the tip of the probe and tapered along a gradient toward distal portions. The chemiluminescence was suppressed significantly by adding either superoxide dismutase (300 U/ml) or sodium azide (20 mmol/l). By adding deuterium to the medium, MCLA-dependent chemiluminescence significantly increased, suggesting the involvement of singlet oxygen in the reaction.