IDENTIFICATION OF PSEUDOMONAS-SYRINGAE PATHOGENS OF ARABIDOPSIS AND A BACTERIAL LOCUS DETERMINING AVIRULENCE ON BOTH ARABIDOPSIS AND SOYBEAN
IDENTIFICATION OF PSEUDOMONAS-SYRINGAE PATHOGENS OF ARABIDOPSIS AND A BACTERIAL LOCUS DETERMINING AVIRULENCE ON BOTH ARABIDOPSIS AND SOYBEAN
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DOI：
10.1105/tpc.3.1.49
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发表时间：
1991-01-01
期刊：
影响因子：
11.6
通讯作者：
STASKAWICZ, BJ
中科院分区：
文献类型：
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作者：
WHALEN, MC;INNES, RW;STASKAWICZ, BJ
为了建立植物-病原菌互作的分子遗传分析模型，研究了拟南芥（Arabidopsis thaliana）与病原菌番茄假单胞菌（Pseudomonasseringae pv tomato，Pst）的互作关系。 Pst菌株被发现是有毒或无毒的特定的拟南芥生态型，和单一的生态型是抗一些Pst菌株和敏感的人。 在许多植物-病原体互作中，抗病性是由单一植物抗病基因和单一病原体无毒基因同时存在所控制的。 因此，我们测试是否无毒基因在Pst控制诱导拟南芥的抗性。 从Pst基因组文库中分离确定无毒力的基因座，并定义Pst无毒力位点avrRpt 2。 这使我们能够构建仅因存在或不存在单一假定无毒基因而不同的病原体。 我们发现拟南芥生态型Col-0对Pst菌株DC 3000敏感，但对携带avrRpt 2的相同菌株具有抗性，这表明Col-0中的单个位点决定抗性。 作为对假定的抗性基因座进行遗传作图的第一步，鉴定了对携带avrRpt 2的DC 3000感染易感的生态型。 还将来自Pst的avrRpt 2基因座移入大豆病原体大豆疫霉致病变种的强毒株中，以测试该基因座是否可以确定对大豆的无毒力。 由此产生的菌株诱导的品种特异性的方式，表明类似的抗性机制可能在拟南芥和大豆的抗性反应。
To develop a model system for molecular genetic analysis of plant-pathogen interactions, we studied the interaction between Arabidopsis thaliana and the bacterial pathogen Pseudomonas syringae pv tomato (Pst). Pst strains were found to be virulent or avirulent on specific Arabidopsis ecotypes, and single ecotypes were resistant to some Pst strains and susceptible to others. In many plant-pathogen interactions, disease resistance is controlled by the simultaneous presence of single plant resistance genes and single pathogen avirulence genes. Therefore, we tested whether avirulence genes in Pst controlled induction of resistance in Arabidopsis. Cosmids that determine avirulence were isolated from Pst genomic libraries, and the Pst avirulence locus avrRpt2 was defined. This allowed us to construct pathogens that differed only by the presence or absence of a single putative avirulence gene. We found that Arabidopsis ecotype Col-0 was susceptible to Pst strain DC3000 but resistant to the same strain carrying avrRpt2, suggesting that a single locus in Col-0 determines resistance. As a first step toward genetically mapping the postulated resistance locus, an ecotype susceptible to infection by DC3000 carrying avrRpt2 was identified. The avrRpt2 locus from Pst was also moved into virulent strains of the soybean pathogen P. syringae pv glycinea to test whether this locus could determine avirulence on soybean. The resulting strains induced a resistant response in a cultivar-specific manner, suggesting that similar resistance mechanisms may function in Arabidopsis and soybean.