Detection of asymptomatic initial herpes simplex virus (HSV) infections in animals immunized with subunit HSV glycoprotein vaccines.
Detection of asymptomatic initial herpes simplex virus (HSV) infections in animals immunized with subunit HSV glycoprotein vaccines.
复制标题
检测用亚单位 HSV 糖蛋白疫苗免疫的动物的无症状初始单纯疱疹病毒 (HSV) 感染。
DOI：
10.1128/jcm.28.1.11-15.1990
复制
发表时间：
1990
影响因子：
9.4
通讯作者：
Myers,MG
中科院分区：
文献类型：
--
作者：
Bernstein,DI;Ashley,RL;Stanberry,LR;Myers,MG
单纯疱疹病毒 (HSV) 疫苗功效的评估将需要检测无症状 HSV 感染并评估这些患者复发风险的方法。感染 HSV 的疫苗接种者应产生针对亚单位疫苗中未包含的 HSV 多肽的抗体。在疫苗接种后但HSV攻击前以及HSV攻击后30天收集来自57只生殖器HSV 2型攻击后无症状初始感染的HSV糖蛋白疫苗接种豚鼠的血清，以确定对HSV多肽的抗体反应。在这 57 只动物中的 32 只 (56%) 进行病毒攻击后收集的血清中检测到了针对非疫苗 HSV 多肽的抗体。 32 只具有可检测抗体的动物中，有 26 只 (81%) 出现复发性疾病；然而，其余 25 例中的 11 例（44%）也出现了复发，这些病例未显示出可检测到的针对非疫苗 HSV 多肽的抗体。与那些产生抗体的动物相比，在攻击后的初始疾病期间，未产生针对非疫苗多肽的抗体的动物中阴道病毒脱落的程度显着较低(病毒脱落曲线下面积，5.2+/-3.2对比18.1+/-5.8；P小于0.0001)。这些数据表明，检测针对非疫苗HSV多肽的抗体将识别出大多数（70%）最初无症状的疫苗接种者，这些疫苗接种者会出现复发性疾病，但即使病毒复制水平显着降低（不诱导可检测的抗体反应），也可以确定潜伏期。
The evaluation of herpes simplex virus (HSV) vaccine efficacy will require methods to detect asymptomatic acquisition of HSV infection and to assess the risk of recurrences in these patients. HSV-infected vaccinees should develop antibodies to HSV polypeptides not included in subunit vaccines. Sera from 57 HSV glycoprotein-vaccinated guinea pigs that had asymptomatic initial infections after genital HSV type 2 challenge were collected after vaccination but before HSV challenge and again 30 days after HSV challenge to determine the antibody response to HSV polypeptides. Antibodies to nonvaccine HSV polypeptides were detected in sera collected after viral challenge from 32 (56%) of these 57 animals. Twenty-six (81%) of the 32 animals with detectable antibody developed recurrent disease; however, recurrences also developed in 11 (44%) of the remaining 25 that did not show detectable antibody to nonvaccine HSV polypeptides. The magnitude of vaginal viral shedding during the initial disease period following challenge was significantly lower in animals that did not develop antibody to nonvaccine polypeptides compared with those that did develop antibody (area under the viral shedding curve, 5.2 +/- 3.2 versus 18.1 +/- 5.8; P less than 0.0001) . These data suggest that detection of antibody to nonvaccine HSV polypeptides will identify the majority (70%) of initially asymptomatic vaccinees that develop recurrent disease but that latency can be established even with markedly reduced levels of viral replication that did not induce a detectable antibody response.