Identification and functional characterization of muscle satellite cells in Drosophila.
Identification and functional characterization of muscle satellite cells in Drosophila.
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DOI：
10.7554/elife.30107
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发表时间：
2017-10-26
期刊：
影响因子：
7.7
通讯作者：
VijayRaghavan K
中科院分区：
文献类型：
--
作者：
Chaturvedi D;Reichert H;Gunage RD;VijayRaghavan K
对果蝇和小鼠等遗传模型系统的研究表明，脊椎动物和无脊椎动物的肌肉发生的基本机制非常相似。然而，引人注目的是，卫星细胞，即脊椎动物受损肌肉再生所必需的成体肌肉干细胞，尚未在无脊椎动物中报道。在这项研究中，我们表明，肌肉干细胞的直系后代存在于果蝇的成年肌肉中，在表面观察到的小的，未融合的细胞，并在接近成熟的肌肉纤维。通常静止，肌纤维损伤后，我们表明，这些细胞表达Zfh 1和从事Notch-Delta依赖性增殖活动，并产生直系后代群体，与受伤的肌纤维融合。鉴于惊人相似的形态和功能特征，我们认为这些新型细胞相当于果蝇的脊椎动物肌肉卫星细胞。
Work on genetic model systems such as Drosophila and mouse has shown that the fundamental mechanisms of myogenesis are remarkably similar in vertebrates and invertebrates. Strikingly, however, satellite cells, the adult muscle stem cells that are essential for the regeneration of damaged muscles in vertebrates, have not been reported in invertebrates. In this study, we show that lineal descendants of muscle stem cells are present in adult muscle of Drosophila as small, unfused cells observed at the surface and in close proximity to the mature muscle fibers. Normally quiescent, following muscle fiber injury, we show that these cells express Zfh1 and engage in Notch-Delta-dependent proliferative activity and generate lineal descendant populations, which fuse with the injured muscle fiber. In view of strikingly similar morphological and functional features, we consider these novel cells to be the Drosophila equivalent of vertebrate muscle satellite cells.