Determination of basic azaarenes in aviation kerosene by solid-phase extraction and HPLC-fluorescence detection.
Determination of basic azaarenes in aviation kerosene by solid-phase extraction and HPLC-fluorescence detection.
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DOI：
10.1002/jssc.200900040
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发表时间：
2009-06
影响因子：
3.1
通讯作者：
Elaine Rocha da Luz;Thiago Fernando Mota Gonsalves;R. Aucélio
中科院分区：
文献类型：
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作者：
Elaine Rocha da Luz;Thiago Fernando Mota Gonsalves;R. Aucélio
SPE 与 HPLC 和荧光检测相结合，可灵敏测定航空煤油（喷气发动机）中的六种碱性氮杂芳烃（7,8-苯并喹啉、7,9-二甲基苯并[c]吖啶、9-氨基-1,2,3,4-四氢吖啶、9-甲基吖啶、吖啶和二苯并[a,j]吖啶） 燃料）。使用强阳离子交换吸附剂一步进行 SPE。 HPLC 系统由 C18 柱和选定的最佳 lambda(exc) 和 lambda(em) 检测程序组成。使用 ACN 和磷酸盐缓冲液 (pH 6.5) 以 1 mL/min 的流速进行梯度洗脱，可在 15 分钟内有效、快速地分离氮杂芳烃。每次进样的 LOD 和 LOQ 值（信噪比分别为 3:1 和 10:1）在 0.0013 至 0.021 之间以及 0.0044 至 0.072 ng 之间。校准曲线显示从 LOQ 到 250 microg/L (r2 >0.99) 的线性行为。对于 6.0 微克/升的加标浓度，喷气燃料样品的回收率为 92% 至 107%，但 9-氨基-1,2,3,4-四氢吖啶除外，其回收率为 68%。所提出的方法适用于一种商业煤油和三种航空煤油样品中这六种基本氮杂芳烃的定量。航空煤油中确认存在 7,8-苯并喹啉（高达 3.2 微克/升）和二苯并[a,j]吖啶（高达 6.3 微克/升）。
SPE in combination with HPLC and fluorescence detection has been used for sensitive determination of six basic azaarenes (7,8-benzoquinoline, 7,9-dimethylbenz[c]acridine, 9-amino-1,2,3,4-tetrahydroacridine, 9-methylacridine, acridine, and dibenz[a,j]acridine) in aviation kerosene (jet fuel). SPE was performed in a single step using a strong cation exchange sorbent. The HPLC system consisted of C18 column with a selected detection program of optimal lambda(exc) and lambda(em). A gradient elution with ACN and phosphate buffer (pH 6.5) at a flow rate of 1 mL/min allowed efficient and fast separation of azaarenes within 15 min. The LOD and LOQ values (S/N ratio 3:1 and 10:1, respectively) were between 0.0013 and 0.021 and from 0.0044 to 0.072 ng per injection. The calibration curves showed linear behavior from the LOQ to 250 microg/L (r2 >0.99). For the spiked concentration of 6.0 microg/L, recoveries were from 92 to 107% for jet fuel samples, except for 9-amino-1,2,3,4-tetrahydroacridine, which presented 68% recovery. The proposed method was applied to the quantification of those six basic azaarenes in one commercial kerosene and in three aviation kerosene samples. The presence of 7,8-benzoquinoline (up to 3.2 microg/L) and dibenzo[a,j]acridine (up to 6.3 microg/L) was confirmed in aviation kerosene.