Comparison of a manual walking platform and the CatWalk gait analysis system in a rat osteoarthritis model
Comparison of a manual walking platform and the CatWalk gait analysis system in a rat osteoarthritis model
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DOI：
10.17219/acem/137536
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发表时间：
2021-09-01
影响因子：
2.1
通讯作者：
Ugurlu, Mahmut
中科院分区：
文献类型：
--
作者：
Kara, Halil;Caglar, Ceyhun;Ugurlu, Mahmut
背景使用不同的步态分析系统在实验动物模型中观察骨关节炎（OA）的影响。目标.本研究旨在确定Noldus CatWalk XT v. 10.9步态分析系统（CatWalk）器械是否可有效用于化学诱导大鼠OA模型，并揭示该系统与手动步态分析相比的优势和劣势。材料和方法。10只Wistar大鼠在手动行走平台以及CatWalk上跑步，并记录基础值。对于OA诱导，在麻醉下向所有大鼠的左膝注射碘乙酸盐（MIA）。在OA发展4周后，再次在手动和CatWalk步态平台上运行大鼠。对于手动步态分析，在两个膝盖上测量步幅长度、爪印宽度和爪印长度。除这些参数外，使用CatWalk设备测量左膝（受影响的膝关节）的平均跑步速度、跑步持续时间、最大接触强度、爪印面积、平均站立和摆动速度。结果通过手动行走平台在OA之前和之后记录的步幅宽度（p = 0.0272）、左步幅长度（p = 0.0344）和左爪印长度（p = 0.0233）中观察到显著差异。对于猫步，基于OA前后获得的数据，检测到左膝的平均跑步速度（p = 0.0010）、最大接触强度（p = 0.0155）、爪印长度（p = 0.0058）、爪印宽度（p = 0.0324）和摆动速度（p = 0.0066）存在显著差异。结论. CatWalk步态分析系统适用于OA大鼠模型和相关干预措施的评价。与手动系统相比，它还提供了额外的参数，并最大限度地减少了与人为相关的变化。
Background. Effects of osteoarthritis (OA) are observed in experimental animal models using different gait analysis systems. Objectives. The aim of this study was to determine whether the Noldus CatWalk XT v. 10.9 gait analysis sys-tem (CatWalk) device can be used effectively in a chemically induced rat OA model and to reveal the strengths and weaknesses of the system compared to manual gait analysis. Materials and methods. Ten Wistar rats were run on a manual walking platform as well as on the CatWalk and the basal values were recorded. For OA induction, monosodium iodoacetate (MIA) was injected into the left knee of all rats under anesthesia. After a period of 4 weeks for OA development, the rats were again run on both the manual and CatWalk gait platforms. For manual gait analysis, the stride length, paw print width and paw print length were measured on both knees. In addition to these parameters, the average run speed, run duration, maximum contact intensity, paw print area, mean stance, and swing speed were measured on the left knee (affected knee) using the CatWalk device. Results. Significant differences were observed in the stride width (p = 0.0272), left stride length (p = 0.0344), and left paw print length (p = 0.0233) recorded before and after OA via the manual walking platform. For CatWalk, a significant difference was detected in the left knee's average run speed (p = 0.0010), maximum contact intensity (p = 0.0155), paw print length (p = 0.0058), paw print width (p = 0.0324), and swing speed (p = 0.0066) based on data obtained before and after OA. Conclusions. The CatWalk gait analysis system is suitable for the evaluation of OA rat models and related interventions. It also provides additional parameters compared to the manual system and minimizes human-related variation.