Ovarian follicular changes and hemodynamics in Egyptian buffaloes under CIDR-PGF2α and Ovsynch-CIDR estrus synchronization treatments
Ovarian follicular changes and hemodynamics in Egyptian buffaloes under CIDR-PGF2α and Ovsynch-CIDR estrus synchronization treatments
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DOI：
10.1262/jrd.2019-035
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发表时间：
2019-10-01
影响因子：
1.8
通讯作者：
Watanabe, Gen
中科院分区：
文献类型：
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作者：
Samir, Haney;Kandiel, Mohamed M. M.;Watanabe, Gen
本研究旨在探讨两种同期发情方案对卵泡变化和血流动力学的影响。多产埃及水牛（n = 36）同步使用药物控释（CIDR）-PGF 2 α（7天CIDR插入，第6天注射PGF 2 α; n = 18）或Ovsynch-CIDR（Ovsynch方案与7天CIDR插入同时进行; n = 18）。在CIDR取出当天、发情期和黄体期进行血样采集和卵巢超声检查（灰度、彩色和能量多普勒模式）。在CIDR停药时，CIDR-PGF 2 α组的平均卵泡直径（MFD）、第一（1st-LF）和第二（2nd-LF）大卵泡直径以及E2水平显著增加。Ovsynch-CIDR显著增加了发情期卵泡数、MFD和第一LF直径以及黄体期黄体体积，同时E2（发情期）和P4（黄体期）增加（P < 0.05）。在去除CIDR时，Ovsynch-CIDR中优势卵泡（DF）中的蓝色像素高于CIDR-PGF 2 α（1.5倍; P = 0.054）。在发情期，总血流量（TBF）和功率多普勒像素（PDP）与DF（s）的比值明显高于对照组（P <0.05）。Ovsynch-CIDR比CIDR-PGF 2 α高7倍和1.6倍（分别为5906 +/-237对830 +/-60像素，P < 0.01和5479 +/-322对3377 +/-19像素，P < 0.05）。在黄体期，Ovsynch-CIDR组的TBF和PDP与CL的比值比CIDR-PGF 2 α组增加（分别为11060 +/- 965 vs. 7963 +/- 480像素，1.4倍，P = 0.05和18900 +/- 1350 vs. 13220 +/- 568像素，1.1倍，P = 0.005）。总之，基于同步卵泡活动和血流动力学的改善，Ovsynch-CIDR方案在埃及水牛中被说服。
This study aimed at investigating the efficacy of two protocols of estrous synchronization on follicular changes and hemodynamics. Pluriparous Egyptian buffaloes (n = 36) were synchronized either with controlled internal drug release (CIDR)-PGF2 alpha (7-days CIDR insert with PGF2 alpha injected on the 6th day; n = 18) or Ovsynch-CIDR (Ovsynch protocol concurrent with 7-days CIDR insert; n = 18). Blood sampling and ovarian ultrasound examinations (Grayscale, color and power Doppler modes) were conducted on the Day of CIDR removal, estrus, and luteal phase. Mean follicle diameter (MFD), first (1st-LF) and second (2nd-LF) largest follicle diameters, and E2 levels significantly increased in the CIDR-PGF2 alpha group at CIDR withdrawal. Ovsynch-CIDR markedly fortified higher follicle population, MFD, and 1st-LF diameter at estrus and corpus luteum (CL) volume at the luteal phase in concomitant with increases (P < 0.05) in E2 (at estrus) and P4 (at luteal phase). At CIDR removal, the blue pixels in the dominant follicle (DF) were higher (1.5 times; P = 0.054) in the Ovsynch-CIDR than in the CIDR-PGF2 alpha. At estrus, total blood flow (TBF) and power Doppler pixels (PDP) to DF(s) were noticeably higher (seven and 1.6 times; respectively) in the Ovsynch-CIDR than in CIDR-PGF2 alpha (5906 +/- 237 vs. 830 +/- 60 pixels, P < 0.01 and 5479 +/- 322 vs. 3377 +/- 19 pixels, P < 0.05; respectively). At the luteal phase, TBF and PDP to the CL increased in the Ovsynch-CIDR group than in the CIDR-PGF2 alpha group (11060 +/- 965 vs. 7963 +/- 480 pixels, 1.4 times, P = 0.05 and 18900 +/- 1350 vs. 13220 +/- 568 pixels, 1.1 times, P = 0.005; respectively). In conclusion, based on the improvement in synchronized follicular activity and hemodynamics, the Ovsynch-CIDR regimen is persuaded in Egyptian buffaloes.