Prenatal testosterone exposure worsen the reproductive performance of male rat at adulthood.
Prenatal testosterone exposure worsen the reproductive performance of male rat at adulthood.
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DOI：
10.1371/journal.pone.0071705
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Azizi F
中科院分区：
文献类型：
--
作者：
Ramezani Tehrani F;Noroozzadeh M;Zahediasl S;Ghasemi A;Piryaei A;Azizi F
生殖系统非常容易受到环境的损害，例如在妊娠发育和分化期间的外源性类固醇。在雌性动物模型中，产前雄激素过量的实验诱导重新编程了它们的生殖生理，然而雄激素过量对雄性生殖系统的胎儿编程尚未得到很好的研究。我们的目的是确定两种不同剂量的睾丸激素产前暴露在不同的妊娠天数，对男性生殖系统的影响，使用大鼠模型。将16只孕鼠随机分为2个实验组和2个对照组。实验组І在妊娠第16 ~ 19天皮下注射游离睾酮3 mg，对照组在妊娠第16 ~ 19天皮下注射溶剂；试验组П在妊娠第20天皮下注射游离睾酮20 mg，对照组同时注射溶剂。比较了各研究组32只雄性后代在6 ~ 30 ~ 60天和青春期后的生殖系统形态和功能。与对照组相比，试验组雄性后代在不同测量天数的肛门生殖器距离无显著差异。试验组І子代青春期后睾丸重量、睾托细胞、精母细胞和精母细胞数量、精子数量、活动力和血清睾酮浓度均显著降低；除精子活力降低（p< 0.01）外，试验组后代无其他影响ІІ。总之，我们的数据表明，雄性大鼠胎儿在产前暴露于过量的睾丸激素会破坏生殖功能，这种影响高度依赖于暴露的时间、持续时间和水平。似乎在胎儿时期暴露于高水平雄激素的个体的生殖系统应该在青春期进行评估，并可能进行治疗。
The reproductive system is extremely susceptible to environmental insults, for example exogenous steroids during gestational development and differentiation. Experimental induction of androgen excess during prenatal life in female animal models reprograms their reproductive physiology, however the fetal programming of the male reproductive system by androgen excess has not been well studied. We aimed to determine the effect of prenatal exposure of two different doses of testosterone on different gestational days, on the male reproductive system using a rat model. Sixteen pregnant rats were randomly divided into two experimental groups and two control groups. Experimental group І were subcutaneously injected with 3 mg free testosterone on gestational days 16-19 and its controls received solvent for that time; experimental group П were subcutaneously injected with 20 mg free testosterone on day 20 of gestational period and its controls received solvent at the same time. The reproductive system morphology and function of 32 male offspring of these study groups were compared at days 6-30-60 of age and after puberty. The anogenital distance of the male offspring of both experimental groups had no significant differences on the different days of measurement, compared with controls. In the offspring of experimental group І, the testes weight, number of Sertoli, Spermatocyte and Spermatid cells, sperm count and motility and the serum concentration of testosterone after puberty were significantly decreased; except for reduction of sperm motility (p< 0.01), the other effects were not observed in the offspring of experimental group ІІ. In summary, our data show that prenatal exposure of male rat fetuses to excess testosterone disrupted reproductive function, an effect highly dependent on the time, duration and level of exposure. It seems that the reproductive system in individuals exposed to high levels of androgens during fetal life should be evaluated at puberty and likely to be treated.
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影响因子：
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作者：
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通讯作者：
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作者：
Amalfi S;Velez LM;Heber MF;Vighi S;Ferreira SR;Orozco AV;Pignataro O;Motta AB
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