Integrated metabolomics and transcriptome analysis on flavonoid biosynthesis in safflower (Carthamus tinctorius L.) under MeJA treatment
Integrated metabolomics and transcriptome analysis on flavonoid biosynthesis in safflower (Carthamus tinctorius L.) under MeJA treatment
复制标题
MeJA 处理下红花 (Carthamustinctorius L.) 类黄酮生物合成的综合代谢组学和转录组分析
DOI：
10.1186/s12870-020-02554-6
复制
发表时间：
2020-07-29
影响因子：
5.3
通讯作者：
Pei, Jin
中科院分区：
文献类型：
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作者：
Chen, Jiang;Wang, Jie;Pei, Jin
背景
红花（红花）是一种重要的经济作物，其中干燥的管花不仅是染料和化妆品的重要原料，也是中药中广泛使用的重要草药。红花的色素和生物活性物质主要由黄酮类化合物（主要是醌类查尔酮）组成，研究表明MeJA能促进醌类查尔酮的生物合成，但其作用机制尚不清楚。本研究试图利用代谢组学和转录组学技术分析MeJA处理下红花类黄酮生物合成的分子机制。
结果
基于UHPLC-ESI-MS/MS检测平台和自建数据库（含羟基茉莉黄A，HSYA），共检测到209种黄酮类代谢产物，其中35种代谢产物经MeJA处理后差异显著。其中，24种代谢产物在MeJA处理后上调，尤其是HSYA。11种代谢产物在MeJA处理后下调。综合代谢组学和转录组学分析表明，MeJA可能通过上调类黄酮生物合成途径上游基因（如CHSs、CHIs和HCTs）的表达，下调下游基因（如F3 Ms、ANRs和ANSs）的表达，从而促进醌类查尔酮（如HSYA）的生物合成。通过实时荧光定量PCR验证这些基因的转录表达。此外，克隆并分析了在MeJA处理下显著上调的两个基因（CtCHI和CtHCT）的启动子。在启动子中分别发现7个和3个MeJA应答元件。
结论
MeJA可能通过上调类黄酮合成途径上游基因的表达，下调下游基因的表达，从而促进醌类查尔酮的合成。本研究结果为茉莉酸甲酯诱导红花黄酮类化合物合成的分子机制研究提供了基础数据。
Background
Safflower (Carthamus tinctorius L.) is an important cash crop, of which the dried tube flower is not only an important raw material for dyes and cosmetics but also an important herb widely used in traditional Chinese medicine. The pigment and bioactive compounds are composed of flavonoids (mainly quinone chalcones), and studies have reported that MeJA can promote the biosynthesis of quinone chalcones, but the mechanism underlying the effect of MeJA in safflower remains unclear. Here, we attempt to use metabolomics and transcriptome technologies to analyse the molecular mechanism of flavonoid biosynthesis under MeJA treatment in safflower.
Results
Based on a UHPLC-ESI-MS/MS detection platform and a self-built database (including hydroxysafflor yellow A, HSYA), a total of 209 flavonoid metabolites were detected, and 35 metabolites were significantly different after treatment with MeJA. Among them, 24 metabolites were upregulated upon MeJA treatment, especially HSYA. Eleven metabolites were downregulated after MeJA treatment. Integrated metabolomics and transcriptome analysis showed that MeJA might upregulate the expression of upstream genes in the flavonoid biosynthesis pathway (such as CHSs, CHIs and HCTs) and downregulate the expression of downstream genes (such as F3Ms, ANRs and ANSs), thus promoting the biosynthesis of quinone chalcones, such as HSYA. The transcription expressions of these genes were validated by real-time PCR. In addition, the promoters of two genes (CtCHI and CtHCT) that were significantly upregulated under MeJA treatment were cloned and analysed. 7 and 3 MeJA response elements were found in the promoters, respectively.
Conclusions
MeJA might upregulate the expression of the upstream genes in the flavonoid biosynthesis pathway and downregulate the expression of the downstream genes, thus promoting the biosynthesis of quinone chalcones. Our results provide insights and basic data for the molecular mechanism analysis of flavonoid synthesis in safflower under MeJA treatment.