Characterization of fission yeast cohesin: essential anaphase proteolysis of Rad21 phosphorylated in the S phase
Characterization of fission yeast cohesin: essential anaphase proteolysis of Rad21 phosphorylated in the S phase
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DOI：
10.1101/gad.832000
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发表时间：
2000-11-01
影响因子：
10.5
通讯作者：
Yanagida, M
中科院分区：
文献类型：
--
作者：
Tomonaga, T;Nagao, K;Yanagida, M
粘着蛋白复合物作用于在S期间和之后姊妹染色单体内聚的形成和维持。萌芽的酵母SCC1P/MCD1P是一个必不可少的亚基，在后期的染色体中分离出来，从而导致姐妹染色质分离。相比之下，较高真核生物中的大多数粘着蛋白在后期之前与染色体分离。因此，粘着蛋白在后期中的普遍作用尚待确定。我们在这里报告了裂变酵母中四个推定的粘蛋白亚基PSM1，PSM3，RAD21和PSC3的初始表征。它们对于姐妹染色单体内聚力至关重要。免疫沉淀表明RAD21与PSM1和PSM3的稳定复合形成，而不是PSC3。染色质免疫沉淀表明，粘蛋白亚基在宽阔的中心粒区域富含，并且与丝粒相关的RAD21的水平没有从中期变为后期，与萌芽的酵母菌截然不同。相反，在后期中特别裂解了与SCC1P/MCD1P相似的裂解位点的RAD21。这种裂解是必不可少的，尽管裂解产物的量很小（
Cohesin complex acts in the formation and maintenance of sister chromatid cohesion during and after S phase. Budding yeast Scc1p/Mcd1p, an essential subunit, is cleaved and dissociates from chromosomes in anaphase, leading to sister chromatid separation. Most cohesin in higher eukaryotes, in contrast, is dissociated from chromosomes well before anaphase. The universal role of cohesin during anaphase thus remains to be determined. We report here initial characterization of four putative cohesin subunits, Psm1, Psm3, Rad21, and Psc3, in fission yeast. They are essential for sister chromatid cohesion. Immunoprecipitation demonstrates stable complex formation of Rad21 with Psm1 and Psm3 but not with Psc3. Chromatin immunoprecipitation shows that cohesin subunits are enriched in broad centromere regions and that the level of centromere-associated Rad21 did not change from metaphase to anaphase, very different from budding yeast. In contrast, Rad21 containing similar cleavage sites to those of Scc1p/Mcd1p is cleaved specifically in anaphase. This cleavage is essential, although the amount of cleaved product is very small(