Comparison of the detection of 3 endangered frog species by eDNA and acoustic surveys across 3 seasons
Comparison of the detection of 3 endangered frog species by eDNA and acoustic surveys across 3 seasons
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DOI：
10.1086/707365
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发表时间：
2020-03
影响因子：
1.8
通讯作者：
T. Takahara;N. Iwai;Kiyomi Yasumiba;Takeshi Igawa
中科院分区：
文献类型：
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作者：
T. Takahara;N. Iwai;Kiyomi Yasumiba;Takeshi Igawa
对濒危物种的保护，基本的生态信息是必不可少的。环境DNA （eDNA）越来越多地用于估计水生生物（如青蛙物种）的存在/缺失。然而，检测eDNA的效率可能取决于目标蛙种的生态特性。本研究采用eDNA方法对3种日本濒危物种Babina subbaspera、Odorrana splendida和O. amamiensis进行了检测。我们在3个不同的季节比较了eDNA检测与传统声学调查方法。两种方法对物种的检测并不总是一致的，可能是因为声学调查只针对成年雄性，而eDNA检测的是两性和水中任何生命史阶段的个体。此外，两种方法在物种间检测模式的差异可能与蝌蚪交配行为特征（如鸣叫量）和生活史特征（如迁徙能力）的不同有关。eDNA法检测的最佳季节在3种昆虫之间也存在差异，这可能与繁殖季节和幼虫特征的差异有关。研究表明，eDNA检测方法和传统的野外观测技术（包括声学调查）的结果并不总是相互一致，而是取决于各自目标物种的生态特征。因此，在应用eDNA方法进行蛙类分布调查时，有必要考虑蛙类的生态特征，如繁殖季节和蝌蚪丰度的季节变化。
For conservation of endangered species, basic ecological information is indispensable. Environmental DNA (eDNA) is increasingly used to estimate the presence/absence of aquatic organisms such as frog species. However, the efficiency of detecting eDNA may depend on the ecological characteristics of the target frog species. Here, we adopted an eDNA approach for detecting 3 endangered Japanese species (Babina subaspera, Odorrana splendida, and O. amamiensis). We compared eDNA detection with a traditional acoustic survey method in 3 different seasons. Detection of species was not always consistent between the 2 methods, probably because acoustic surveys target only calling adult males but eDNA detects individuals of both sexes and at any life-history stage in water. Moreover, the differences in detection patterns between the 2 methods among species may be correlated with different characteristics of mating behavior (e.g., calling volume) and life history of tadpoles (e.g., migration ability). The optimal season for detection by eDNA method also differed between the 3 species, probably because of differences in breeding season and larval characteristics. We show that results obtained from eDNA detection methods and traditional field observation techniques, including acoustic surveys, are not always consistent with each other but rather depend on the ecological characteristics of the respective target species. It is therefore necessary to consider the ecological traits of frog species, such as breeding season and seasonal shifts in tadpole abundance, when applying eDNA methods for distribution surveys.