The Deficient Carbohydrate Metabolic Pathways and the Incomplete Tricarboxylic Acid Cycle in an Obligately Autotrophic Hydrogen-oxidizing Bacterium
The Deficient Carbohydrate Metabolic Pathways and the Incomplete Tricarboxylic Acid Cycle in an Obligately Autotrophic Hydrogen-oxidizing Bacterium
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专性自养氧化氢细菌中碳水化合物代谢途径缺陷和三羧酸循环不完整
DOI：
10.1271/bbb1961.46.2341
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Y. Minoda
中科院分区：
文献类型：
--
作者：
H. Shiba;T. Kawasumi;Y. Igarashi;T. Kodama;Y. Minoda
对极嗜热、专性自养、好氧氧化菌TK-6的无细胞提取物中碳水化合物代谢酶、三羧酸循环酶及相关酶的活性进行了测定。磷酸果糖激酶、醛缩酶、丙酮酸激酶、6-磷酸葡萄糖酸脱水酶和2-酮-3-脱氧-6-磷酸葡萄糖酸醛缩酶是Embden-Meyerhof通路和enterner - doudoroff通路的关键酶，在提取物中未发现活性。除α-酮戊二酸脱氢酶和还原性烟酰胺腺嘌呤二核苷酸氧化酶外，其余三羧酸循环酶均存在。这些代谢缺陷被认为是菌株TK-6专性自养的原因之一。
The activities of enzymes of carbohydrate metabolism, enzymes of the tricarboxylic acid cycle and some related enzymes were measured in cell-free extracts of strain TK-6, an extremely thermophilic, obligately autotrophic, aerobic hydrogen-oxidizing bacterium. Activities of phosphofructokinase, aldolase, pyruvate kinase, 6-phosphogluconate dehydrase and 2-keto-3-deoxy-6-phosphogluconate aldolase, key enzymes of the Embden-Meyerhof and the Entner-Doudoroff pathways were not found in the extracts. All of the tricarboxylic acid cycle enzymes except α-ketoglutarate dehydrogenase, and reduced nicotinamide adenine dinucleotide oxidase were present. These metabolic defects are considered to be one of the reasons for the obligate autotrophy of strain TK-6.