INTERACTIONS OF METAL IONS WITH POLYNUCLEOTIDES AND RELATED COMPOUNDS .4. DEGRADATION OF POLYRIBONUCLEOTIDES BY ZINC AND OTHER DIVALENT METAL IONS
INTERACTIONS OF METAL IONS WITH POLYNUCLEOTIDES AND RELATED COMPOUNDS .4. DEGRADATION OF POLYRIBONUCLEOTIDES BY ZINC AND OTHER DIVALENT METAL IONS
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DOI：
10.1002/bip.360030110
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发表时间：
1965-01-01
期刊：
影响因子：
2.9
通讯作者：
EICHHORN, GL
中科院分区：
文献类型：
--
作者：
BUTZOW, JJ;EICHHORN, GL
聚腺苷酸在64 ℃下20小时内降解为单核苷酸和低分子量寡核苷酸。通过Mn（II）、Co（II）、Ni（II）和Cu（II）离子的作用，以及在2小时内通过Zn（II）离子的作用。后者也以大致相同的速率降解poly C、poly U和RNA，但poly I的降解速度要慢得多。在多核苷酸的碱性降解中没有观察到反应速率的这种差异。与聚I的反应的缓慢性不是由于锌-聚I复合物的任何高度有序的结构，而是可能由于锌-磷酸盐键通过锌与肌苷碱的同时结合而减弱。这一假说的证据来自于肌苷酸或聚I对聚A的锌降解的抑制。在锌和碱降解RNA时，未降解残基的核苷酸组成没有显著差异。
Polyadenylic acid is degraded into mononucleotides and low molecular weight oligo-nucleotides in a 20 hr. period at 64[degree]C. by the action of Mn (II), Co (II), Ni (II), and Cu (II) ions, and in a 2 hr. period by Zn (II) ions. The later also degrade poly C, poly U, and RNA at approximately the same rate, but poly I is degraded very much more slowly. No such difference in reaction rates can be observed in the alkaline degradation of polynucleotides. The sluggishness of the reaction with poly I is not due to any highly ordered structure of the zinc-poly I complex, but probably to the weakening of the zinc-phosphate bond through simul taneous binding of the zinc to the inosine base. Evidence for such a hypothesis is derived from the inhibition of the zinc degradation of poly A by inosinic acid or poly I. No drastic difference in the nucleotide composition of the undegraded residue can be observed in the degration of RNA by zinc and by alkali.