Construction of a molecular functional map of rapeseed (Brassica napus L.) using differentially expressed genes between hybrid and its parents
Construction of a molecular functional map of rapeseed (Brassica napus L.) using differentially expressed genes between hybrid and its parents
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DOI：
10.1007/s10681-006-9173-9
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发表时间：
2006-11-01
期刊：
影响因子：
1.9
通讯作者：
Li, Chunyan
中科院分区：
文献类型：
--
作者：
Li, Yuanyuan;Ma, Chaozhi;Li, Chunyan
甘蓝型油菜大豆是重要的油料和饲料作物，在种子产量和其他农艺性状上具有显著的杂种优势，但杂种优势的分子基础却知之甚少。作为理解与这一现象相关的分子事件的第一步，利用微阵列鉴定的杂交种中差异表达的基因构建了油菜的分子功能图谱。应用单链构象多态性（SSCP）分析方法，对“SI-1300 ×大麦”杂交F2群体的184个个体进行了遗传作图。共162个标记（154个位点，对应17个功能类别的98个差异表达基因）和8个SSR标记组成21个连锁群，总图距为2267.3cM。随后，将该图谱与拟南芥进行计算机比对。比较图谱显示，位于每条拟南芥染色体上的基因在不同的B中具有直系同源物。甘蓝型油菜LGS类似地，大多数LG由来自不同拟南芥染色体的同源基因组成。此外，在B中共鉴定出25个同线区域。napus中的基因序列，大部分基因序列在两个物种间不一致，A.拟南芥基因组与B中的1-5个不同区域同源。油菜基因组这些结果表明，开发利用A. B的拟南芥信息。napus基于synteny。
Brassica napus L. is an important oilseed and fodder crop with significant heterosis for seed yield and other agronomic traits, but very little is known about the molecular basis of heterosis. As an initial step towards understanding the molecular events associated with this phenomenon, a molecular functional map of rapeseed was constructed using differentially expressed genes in hybrid identified by microarrays. Single-strand conformational polymorphism (SSCP) analysis was applied for genetic mapping in an F-2 population of 184 individuals resulting from crossing "SI-1300 x Eagle''. A total of 162 markers including 154 loci corresponding to 98 differentially expressed genes assigned to 17 functional categories and 8 SSR markers were grouped into 21 linkage groups (LGs), covering a total map distance of 2267.3 cM. Subsequently, this map was aligned with Arabidopsis thaliana in silico. Comparative mapping shows that genes localized on each Arabidopsis chromosome have orthologs dispreading in different B. napus LGs. Similarly, a majority of LGs were made of homologous genes from different Arabidopsis chromosomes. In addition, a total of 25 syntenic regions were identified in B. napus, in most of which the gene order was not consistent between the two species, and each of the conserved regions in the A. thaliana genome was homologous to 1-5 distinct regions in the B. napus genome. These results indicate that it is not easy to exploit A. thaliana information for B. napus based on synteny.