Cell optoporation with a sub-15 fs and a 250-fs laser
Cell optoporation with a sub-15 fs and a 250-fs laser
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DOI：
10.1117/1.jbo.21.6.060501
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发表时间：
2016-06-01
影响因子：
3.5
通讯作者：
Koenig, Karsten
中科院分区：
文献类型：
--
作者：
Breunig, Hans Georg;Batista, Ana;Koenig, Karsten
我们采用了两种市售的飞秒激光器，一种钛宝石和一种基于镱的振荡器，以在一个常见的实验装置中从用户的实际观点直接比较瞬时激光诱导的细胞膜透化的效率，即，所谓的optoporation。实验装置包括一个修改后的多光子激光扫描显微镜采用高NA聚焦光学。一个自动的细胞照射程序，实现了定制的软件，确定细胞的位置和控制相关的硬件组件。钛：蓝宝石和镱基振荡器分别产生约800 nm和250-fs的脉冲在1044 nm的宽带次15-fs的脉冲。一个更高的optoporation率和后处理的可行性，观察较短的飞秒脉冲，确认多光子效应的重要性，有效optoporation。(C)2016年，美国光电仪器工程师学会（SPIE）
We employed two commercially available femtosecond lasers, a Ti:sapphire and a ytterbium-based oscillator, to directly compare from a user's practical point-of-view in one common experimental setup the efficiencies of transient laser-induced cell membrane permeabilization, i.e., of so-called optoporation. The experimental setup consisted of a modified multiphoton laser-scanning microscope employing high-NA focusing optics. An automatic cell irradiation procedure was realized with custom-made software that identified cell positions and controlled relevant hardware components. The Ti: sapphire and ytterbium-based oscillators generated broadband sub-15-fs pulses around 800 nm and 250-fs pulses at 1044 nm, respectively. A higher optoporation rate and posttreatment viability were observed for the shorter fs pulses, confirming the importance of multiphoton effects for efficient optoporation. (C) 2016 Society of Photo-Optical Instrumentation Engineers (SPIE)