Preparation and Oxidation Stability Evaluation of Tea Polyphenols-Loaded Inverse Micro-Emulsion
Preparation and Oxidation Stability Evaluation of Tea Polyphenols-Loaded Inverse Micro-Emulsion
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DOI：
10.1111/1750-3841.13689
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发表时间：
2017-05-01
影响因子：
3.9
通讯作者：
Wang, Zhengwu
中科院分区：
文献类型：
--
作者：
Lan, Xiaohong;Sun, Jingjing;Wang, Zhengwu
与合成抗氧化剂相比，茶多酚（TPs）在食用油工业中有其自身的优势，但其亲水性限制了其应用。本研究构建了单油酸甘油酯（GMO）、吐温80、亚油酸为表面活性剂，乙醇为助表面活性剂，大豆油、玉米油、葵花籽油为油相的负载TPs的微乳液（ME）体系的三元相图。结果表明，ME组合物（57.5%油、18% Tween80、18% GMO、4%亚油酸和2.5%水+乙醇）可以溶解最大量的水，并且通过动态光散射（DLS）检测，可以在室温下稳定2个月，平均直径为6至7 nm。由于水相极性的增加，将 TP 加载到 ME 中导致颗粒尺寸增加到 15 至 16 nm。通过过氧化值（POV）方法表征ME中TPs的抗氧化能力。添加0.1 g/mL TPs的1%水相在加速温度50℃下可将POV保持在较低值30 d。同时，与三种食用油相比，ME和玉米油在储存期间具有较低的电导率和较高的POV值。该工作为TPs负载ME的制备提供了一种高效、环境友好的方法，有利于TPs在食用油中的应用。
Compared to synthetic antioxidants, tea polyphenols (TPs) has its own advantages in edible oil industry, however, the hydrophilic properties have restricted its applications. In this study, the ternary phase diagram of TPs-loaded micro-emulsion (ME) system was constructed, in which glyceryl monooleate (GMO), Tween80, linoleic acid as the surfactants, ethanol as the co-surfactant and soybean, corn, sunflower oil as the oil phase, have been used for the preparation of ME. The results indicated that a composition of ME (57.5% oil, 18% Tween80, 18% GMO, 4% Linolic acid, and 2.5% water+ethanol) could dissolve maximum water and could stable for 2 mo at room temperature with an average diameter of 6 to 7 nm, as detected by means of dynamic light scattering (DLS). The loaded of TPs into ME led to an increase of particle size to 15 to 16 nm, due to increased polarity of the water phase. The antioxidant capacity of TPs in ME was characterized by the peroxide value (POV) method. The addition of 1% water phase with 0.1 g/mL TPs could retain the POV at low value for 30 d at accelerating temperature 50 degrees C. Meanwhile, comparing the three edible oil, ME with corn oil has lower conductivity and higher value of POV during the storage. This work provides an efficient and environmentally friendly approach for the preparation of TPs-loaded ME, which is beneficial to the application of TPs in edible oil.