Feedback-Regulation of Strigolactone Biosynthetic Genes and Strigolactone-Regulated Genes in Arabidopsis
Feedback-Regulation of Strigolactone Biosynthetic Genes and Strigolactone-Regulated Genes in Arabidopsis
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DOI：
10.1271/bbb.90443
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发表时间：
2009-11-01
影响因子：
1.6
通讯作者：
Asami, Tadao
中科院分区：
文献类型：
--
作者：
Mashiguchi, Kiyoshi;Sasaki, Eriko;Asami, Tadao
独脚金内酯（SL）最近被发现调节芽分支，但SL在其他发育阶段的功能和SL相关基因的表达调控大多是未知的拟南芥。在这项研究中，我们进行了实时逆转录-PCR（RT-PCR）和微阵列分析使用野生型植物和SL-缺陷/不敏感突变体，以了解SL生物合成和信号转导的分子机制。我们发现拟南芥幼苗的基因表达对SL有反应性，其中包括两个类胡萝卜素裂解双加氧酶基因的反馈调节。基因芯片分析表明，外源SL调控几个基因的表达，包括光信号相关基因和生长素诱导基因。我们还发现MORE AXILLARY GROWTH（MAX）2在SL调节基因的表达中起重要作用。我们的数据支持以前的研究表明，SL可能在幼苗阶段发挥作用。SL响应和MAX2下游候选基因的分析提供了新的机会，以扩大我们对SL信号的理解。
Strigolactones (SLs) have recently been found to regulate shoot branching, but the functions of SLs at other stages of development and the regulation of SL-related gene expression are mostly unknown in Arabidopsis. In this study, we performed real-time reverse transcription-PCR (RT-PCR) and microarray analysis using wild-type plants and SL-deficient/insensitive mutants to understand the molecular mechanisms underlying SL biosynthesis and signaling. We found that there is responsiveness to SL in the gene expression of Arabidopsis seedlings, which includes feedback regulation of two carotenoid cleavage dioxygenase genes. Microarray analysis revealed that exogenously applied SL regulated the expression of several genes, including light signaling-related genes and auxin-inducible genes. We also found that MORE AXILLARY GROWTH (MAX)2 plays an important role in the expression of SL-regulated genes. Our data support previous studies indicating that SL might function at the seedling stage. Analysis of SL-responsive and MAX2 downstream gene candidates provides new opportunities to broaden our understanding of SL signaling.