REGULATION OF CSF COMPOSITION - BLOCKING CHLORIDE-BICARBONATE EXCHANGE
REGULATION OF CSF COMPOSITION - BLOCKING CHLORIDE-BICARBONATE EXCHANGE
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DOI：
10.1152/jappl.1983.55.1.177
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发表时间：
1983-01-01
影响因子：
3.3
通讯作者：
KAZEMI, H
中科院分区：
文献类型：
--
作者：
FRANKEL, H;KAZEMI, H
通过阻断假定的Cl--HCO 3-交换转运系统，研究了呼吸性酸中毒期间观察到的CSF [HCO 3-]增加可能是CSF [Cl-]减少的结果。麻醉犬（戊巴比妥30 mg，i. v.）在侧脑室给予4，4“-二异硫氰基-2，2”-二磺酸芪（DIDS，0.02 μ mol/kg）或吡哆醛5-磷酸（P-5-P，4 μ mol/kg），并维持正常碳酸血症（RA）或高碳酸血症（5%CO2）。测定CSF和动脉PCO 2 [CO2分压]、pH、[Na+]、[Ca 2 +]、[Cl-]和[乳酸盐]。计算[HCO 3-]。在给药前和给药后4 h采集样本。在接受RA的对照犬中，4 h后未观察到任何测量参数的显著变化。接受DIDS或P-5-P和RA的犬CSF [Na+]降低2-4 meq/l，CSF [Cl-]降低6 meq/l。DIDS犬的CSF [HCO 3-]降低5毫克当量/升。P犬CSF [HCO 3-]无明显变化。在5%CO2中4小时后，对照狗的CSF [Cl-]降低3 meq/l，CSF [HCO 3-]增加约4 meq/l。接受P-5-P DIDS并呼吸5%CO2 4小时的犬CSF [Cl-]降低6-9 meq/l，[HCO 3-]升高7-9 meq/l。在任何组的呼吸性酸中毒期间，CSF [Na+]均未发生变化。CSF中的Cl-转运可以通过使用抑制Cl--HCO 3-交换通道的阻断剂来干扰。
The possibility that the increase in CSF [HCO3-] observed during respiratory acidosis could be the result of a decrease in CSF [Cl-] was studied by blocking a putative Cl--HCO3- exchange transport system. Anesthetized dogs (pentobarbital 30 mg, i.v.) were given either 4,4''-diisothiocyano-2,2''-disulfonate stilbene (DIDS, 0.02 .mu.mol/kg) or pyridoxal 5-phosphate (P-5-P,4 .mu.mol/kg) in the lateral cerebral ventricles and maintained either normocapnic (RA) or hypercapnic (5% CO2). CSF and arterial PCO2 [partial pressure of CO2], pH, [Na+], [Ca2+], [Cl-] and [lactate] were determined. [HCO3-] was calculated. Samples were drawn before and 4 h after the drug was administered. In control dogs on RA no significant change was observed in any measured parameter after 4 h. Dogs receiving either DIDS or P-5-P and on RA had decreased CSF [Na+] of 2-4 meq/l and CSF [Cl-] of 6 meq/l. CSF [HCO3-] in DIDS dogs decreased .apprx. 5 meq/l. There was no significant change in CSF [HCO3-] in P dogs. After 4 h of 5% CO2, control dogs had decreased CSF [Cl-] of 3 meq/l and CSF [HCO3-] increased .apprx. 4 meq/l. Dogs receiving DIDS of P-5-P and breathing 5% CO2 for 4 h had decreased CSF [Cl-] of 6-9 meq/l and increased [HCO3-] of 7-9 meq/l. CSF [Na+] did not change during respiratory acidosis in any group. The Cl- transport in CSF can be interfered with by use of blocking agents which inhibit C1--HCO3- exchange channels.