Syntaxin 17 is abundant in steroidogenic cells and implicated in smooth endoplasmic reticulum membrane dynamics
Syntaxin 17 is abundant in steroidogenic cells and implicated in smooth endoplasmic reticulum membrane dynamics
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DOI：
10.1091/mbc.11.8.2719
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发表时间：
2000-08-01
影响因子：
3.3
通讯作者：
Scheller, RH
中科院分区：
文献类型：
--
作者：
Steegmaier, M;Oorschot, V;Scheller, RH
内质网（ER）由具有不同蛋白质成分、形态学外观和功能的亚室组成。为了了解调节内质网复杂和动态组织的机制，重要的是要确定和表征参与组装和维护不同亚室的分子机制。在这里，我们报告，syntaxin 17是丰富的类固醇合成细胞表达。类型，并专门定位于ER的光滑膜。通过免疫沉淀分析，突触融合蛋白17与突触融合蛋白调节蛋白rsly 1和/或两个中间室SNARE蛋白rsec 22 b和rbet 1复合存在。此外，我们发现，突触融合蛋白17锚定的滑面内质网通过一个不寻常的机制，需要两个相邻的疏水结构域附近的羧基末端。越来越多的证据表明，突触融合蛋白17在囊泡运输步骤中起作用，这些囊泡运输步骤到达在类固醇生成细胞中丰富的光滑表面的管状ER膜。
The endoplasmic reticulum (ER) consists of subcompartments that have distinct protein constituents, morphological appearances, and functions. To understand the mechanisms that regulate the intricate and dynamic organization of the endoplasmic reticulum, it is important to identify and characterize the molecular machinery involved in the assembly and maintenance of the different subcompartments. Here we report that syntaxin 17 is abundantly expressed in steroidogenic cell. types and specifically localizes to smooth membranes of the ER. By immunoprecipitation analyses, syntaxin 17 exists in complexes with a syntaxin regulatory protein, rsly1, and/or two intermediate compartment SNARE proteins, rsec22b and rbet1. Furthermore, we found that syntaxin 17 is anchored to the smooth endoplasmic reticulum through an unusual mechanism, requiring two adjacent hydrophobic domains near its carboxyl terminus. Converging lines of evidence indicate that syntaxin 17 functions in a vesicle-trafficking step to the smooth-surfaced tubular ER membranes that are abundant in steroidogenic cells.