Myosin heavy chain mRNA isoforms are expressed in two distinct cohorts during C2C12 myogenesis
Myosin heavy chain mRNA isoforms are expressed in two distinct cohorts during C2C12 myogenesis
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DOI：
10.1007/s10974-011-9267-4
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发表时间：
2012-03-01
影响因子：
2.7
通讯作者：
Brameld, John M.
中科院分区：
文献类型：
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作者：
Brown, David M.;Parr, Tim;Brameld, John M.
肌纤维转变的调节主要是使用常规的组织学或免疫组织化学技术在体内研究的。为了研究细胞培养研究中肌球蛋白重链（MyHC）亚型表达的分子调控，我们首先描述了C2C12肌细胞分化过程中MyHC亚型和生肌调节因子内源性表达的正常转换。有趣的是，在整个分化过程中，MyHC mRNA亚型以不同的时间模式表达为两个不同的队列，一个包括MyHC I，胚胎和新生儿，另一个包括MyHC IIa，IIx和IIb。的表达模式表明，在肌细胞分化过程中，从一个队列到另一个队列，发生在MyHC亚型的过渡，这些过渡发生独立的神经支配。据我们所知，这是最全面的分析，在体外MyHC mRNA亚型转换，并提供了重要的信息，研究在细胞培养系统中的MyHC亚型的转换的调节。
The regulation of muscle fibre transitions has mainly been studied in vivo using conventional histological or immunohistochemical techniques. In order to investigate the molecular regulation of myosin heavy chain (MyHC) isoform expression in cell culture studies, we first characterised the normal transitions in endogenous expression of the MyHC isoforms and the myogenic regulatory factors during differentiation of C2C12 muscle cells. Interestingly, across the time course of differentiation, MyHC mRNA isoforms were expressed in a distinct temporal pattern as two distinct cohorts, one including MyHC I, embryonic and neonatal, the other including MyHC IIa, IIx and IIb. The pattern of expression suggests a transition in MyHC isoforms, from one cohort to another, occurs during muscle cell differentiation and that these transitions occur independent of nerve innervation. To our knowledge, this is the most comprehensive analysis of in vitro MyHC mRNA isoform transitions and provides important information for studying the regulation of transitions in MyHC isoforms in cell culture systems.