Ammonium-induced shoot ethylene production is associated with the inhibition of lateral root formation in Arabidopsis
Ammonium-induced shoot ethylene production is associated with the inhibition of lateral root formation in Arabidopsis
复制标题
铵诱导的芽乙烯产生与拟南芥侧根形成的抑制有关
DOI：
10.1093/jxb/ert019
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发表时间：
2013-03-01
影响因子：
6.9
通讯作者：
Shi, Weiming
中科院分区：
文献类型：
--
作者：
Li, Guangjie;Li, Baohai;Shi, Weiming
叶面接触 NH4 与抑制侧根 (LR) 形成有关。在这里，使用野生型和突变株系研究了拟南芥中芽乙烯在 NH4 诱导的 LR 形成抑制中的作用，这些株系表现出乙烯信号传导被阻断 (etr1) 或乙烯合成增强 (eto1、xbat32)。野生型拟南芥的 NH4 暴露导致主要在远端根中的 LR 产生明显受到抑制，并引发乙烯进化并增强芽中乙烯报告基因 EBS:GUS 的活性。结果表明，枝条与 NH4 的接触对于刺激枝条乙烯的释放是必要的。乙烯拮抗剂 Ag 和氨基乙氧基乙烯基甘氨酸 (AVG) 可减轻 NH4 处理下的 LR 抑制。 eto1-1 和 xbat32 的 LR 产量下降幅度明显更大，而 etr1-3 的 LR 产量下降幅度则明显较小。当在 NH4 存在的情况下施用生长素时，增强的芽乙烯合成/信号传导会阻止 LR 产生的恢复，并对芽 AUX1 表达产生负面影响。这些发现强调了芽乙烯进化在 NH4 介导的 LR 形成抑制中的重要作用。
Foliar NH4 exposure is linked to inhibition of lateral root (LR) formation. Here, the role of shoot ethylene in NH4-induced inhibition of LR formation in Arabidopsis was investigated using wild-type and mutant lines that show either blocked ethylene signalling (etr1) or enhanced ethylene synthesis (eto1, xbat32). NH4 exposure of wild-type Arabidopsis led to pronounced inhibition of LR production chiefly in the distal root, and triggered ethylene evolution and enhanced activity of the ethylene reporter EBS:GUS in the shoot. It is shown that shoot contact with NH4 is necessary to stimulate shoot ethylene evolution. The ethylene antagonists Ag and aminoethoxyvinylglycine (AVG) mitigated LR inhibition under NH4 treatment. The decrease in LR production was significantly greater for eto1-1 and xbat32 and significantly less for etr1-3. Enhanced shoot ethylene synthesis/signalling blocked recovery of LR production when auxin was applied in the presence of NH4 and negatively impacted shoot AUX1 expression. The findings highlight the important role of shoot ethylene evolution in NH4-mediated inhibition of LR formation.