The dynamic character of the G-quadruplex element in the c-MYC promoter and modification by TMPyP4
The dynamic character of the G-quadruplex element in the c-MYC promoter and modification by TMPyP4
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DOI：
10.1021/ja040022b
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发表时间：
2004-07-21
影响因子：
15
通讯作者：
Hurley, LH
中科院分区：
文献类型：
--
作者：
Seenisamy, J;Rezler, EM;Hurley, LH
C-myc基因P1和P2启动子上游的核酸酶超敏元件III1(NHE III1)控制着该基因80-90%的转录活性。该区域富含嘌呤的链可以形成G-四链结构，这是该启动子的沉默元件的关键部分。我们已经证明，这种G-四链结构可以形成四个生物相关的平行环异构体的混合物，当它们与阳离子卟啉TMPyP4相互作用时，可以转化为混合的平行/反平行G-四链结构。
The nuclease hypersensitivity element III1 (NHE III1) upstream of the P1 and P2 promoters of c-MYC controls 80-90% of the transcriptional activity of this gene. The purine-rich strand in this region can form a G-quadruplex structure that is a critical part of the silencer element for this promoter. We have demonstrated that this G-quadruplex structure can form a mixture of four biologically relevant parallel-loop isomers, which upon interaction with the cationic porphyrin TMPyP4 are converted to mixed parallel/antiparallel G-quadruplex structures.