Quantitative evaluation of carbon isotopic fractionation during reversed-phase high-performance liquid chromatography.
Quantitative evaluation of carbon isotopic fractionation during reversed-phase high-performance liquid chromatography.
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反相高效液相色谱过程中碳同位素分馏的定量评估。
DOI：
10.1016/s0021-9673(96)00694-2
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Brenna,JT
中科院分区：
文献类型：
--
作者：
Caimi,RJ;Brenna,JT
对棕榈酸甲酯在低性能制备LC和高性能LC过程中~(13)C的分馏进行了定量研究，并用高精度同位素比值质谱仪(IR-MS)进行了分析。对于制备柱和高性能分析柱，13C浓缩比母体起始物质大约7‰，在峰的第一段急剧下降，然后稳定到比起始物质低约1‰的值。在单一的高效液相色谱柱上循环使用不会引起更大的分离。这些结果强调了定量峰收集对于高精度IR-MS研究的重要性，特别是在峰的第一部分，其中同位素比率变化很快。
The fractionation of13C during low-performance preparative LC and high-performance LC is reported quantitatively for methyl palmitate and using high-precision isotope ratio mass spectrometry (IR-MS). For both preparative and high-performance analytical columns,13C enrichment is about 7‰ greater than the parent starting material, drops sharply in the first section of the peak and then settles to a value about 1‰ below that of the starting material. Recycling over a single HPLC column did not induce greater fractionation. These results emphasize the importance of quantitative peak collection for high-precision IR-MS studies, particularly the first part of the peak where the isotope ratio changes rapidly.