PREPARATION OF 2'(3')-O-ACYL-PCPA DERIVATIVES AS SUBSTRATES FOR T4 RNA LIGASE-MEDIATED CHEMICAL AMINOACYLATION
PREPARATION OF 2'(3')-O-ACYL-PCPA DERIVATIVES AS SUBSTRATES FOR T4 RNA LIGASE-MEDIATED CHEMICAL AMINOACYLATION
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DOI:
10.1016/0040-4020(84)85106-6
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发表时间:
1984-01-01
期刊:
影响因子:
2.1
通讯作者:
HECHT, SM
中科院分区:
文献类型:
--
作者:
HECKLER, TG;CHANG, LH;HECHT, SM
The synthesis of 2''(3'')-O-(amino)acyl-pCpA derivatives is described and the products are characterized chemically and as substrates for T4 RNA ligase in the presence of Escherichia coli tRNAPhe-COH. The modified (N-acetylamino)acyl-tRNA so derived are characterized by their chromatographic and biochemical properties and by their ability to act as P-site tRNA donors in the peptidyltransferase reaction.