DEGRADATION OF DACARBAZINE IN AQUEOUS-SOLUTION
DEGRADATION OF DACARBAZINE IN AQUEOUS-SOLUTION
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DOI：
10.1016/0731-7085(92)80096-6
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发表时间：
1992-09-01
影响因子：
3.4
通讯作者：
RILEY, CM
中科院分区：
文献类型：
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作者：
SHETTY, BV;SCHOWEN, RL;RILEY, CM
在37℃条件下考察了初始浓度(0.05-5.0 mg ml-1, 2.5 x 10(-4)-0.025 M)(pH 1-13)、缓冲液浓度(0.01-0.075 M)、光、抗氧化剂和共溶剂对达卡巴嗪水溶液降解的影响。使用液相色谱法监测达卡巴嗪的降解以及降解产物的出现。达卡巴嗪在黑暗中的水解动力学是准一级的并且与药物的初始浓度无关。光加速了达卡巴嗪的降解，并且在低浓度下通过伪零级动力学进行。 pH 速率曲线表明光解反应和水解反应均取决于分子的电离状态。通过液相色谱检测并通过质谱确认水解和光解的主要降解产物是2-氮杂次黄嘌呤。
The effects of initial concentration (0.05-5.0 mg ml-1, 2.5 x 10(-4)-0.025 M) (pH 1-13), buffer concentration (0.01-0.075 M), light, antioxidants and co-solvents on the degradation of dacarbazine in aqueous solution were investigated at 37-degrees-C. Liquid chromatography was used to monitor the degradation of dacarbazine as well as the appearance of degradation products. The kinetics of hydrolysis of dacarbazine in the dark were pseudo first-order and independent of the initial concentration of the drug. The degradation of dacarbazine was accelerated by light and at low concentration proceeded by pseudo zero-order kinetics. The pH-rate profiles showed that both the photolytic and the hydrolytic reactions were dependent on the ionization state of the molecule. The main degradation product of both hydrolysis and photolysis was detected by liquid chromatography and confirmed by mass spectrometry to be 2-azahypoxanthine.