Molecular identification of blood source animals from black flies (Diptera: Simuliidae) collected in the alpine regions of Japan
Molecular identification of blood source animals from black flies (Diptera: Simuliidae) collected in the alpine regions of Japan
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DOI：
10.1007/s00436-009-1667-7
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发表时间：
2010-01-01
影响因子：
2
通讯作者：
Yukawa, Masayoshi
中科院分区：
文献类型：
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作者：
Imura, Takayuki;Sato, Yukita;Yukawa, Masayoshi
洛氏白细胞虫（Leucocytozoon lovati）是一种媒介传播的鸟类原生动物，在分布于日本高山地区的濒危鸟类松鸡（Lagopus mutus）体内发现。传病节肢动物L. lovati也被估计为蚋科黑蝇，分布在宿主鸟类的同一栖息地，然而，尚未确定黑蝇的可能血餐。为了揭示黑蝇的宿主动物，我们利用分子生物学技术对黑蝇的血液资源进行了估算。黑蝇在Mt. 2005年6月，日本高山地区之一的长岳，日本岩雷鸟居住的地方。经形态学鉴定，144个标本分为日本蚋87个种，毛足原蚋48个种，条斑原蚋3个种，双纹螺3个种，变纹螺3个种。从黑蝇中单独提取的DNA进行聚合酶链反应（PCR）扩增，靶向鸟类或哺乳动物的部分线粒体细胞色素B基因，以鉴定血粉。在144份黑蝇标本中，34份为禽源宿主PCR阳性，阳性率为23.6%。从研究的样本中未检测到任何来源的血液。从11只黑蝇中扩增出的序列包括S. japonicum、毛足拟青霉（P.hirtipes）和毛足拟青霉（C.穆塔塔与日本岩雷鸟相似性较高。因此，本研究结果最终表明，这三种黑蝇可能吸食日本岩雷鸟的血液，并可能成为日本岩雷鸟的媒介。日本的这种濒危鸟类中的lovati感染。
One of vector-borne avian protozoa, Leucocytozoon lovati, has been found in the Japanese rock ptarmigans (Lagopus mutus japonicus), the endangered bird species distributed in the alpine regions in Japan. Vector arthropod species of L. lovati has also been estimated as Simuliidae black flies distributed in the same habitat of the host bird, however, possible blood meals of the black flies were not identified yet. To reveal host animals of black flies, we estimated the blood resources by using molecular techniques. Black flies were collected at Mt. Chogatake, one of the alpine regions of Japan in which Japanese rock ptarmigans live in June 2005. The analyzed 144 specimens were morphologically identified into five species including Simulium japonicum (n = 87), Prosimulium hirtipes (n = 48), Prosimulium yezoense (n = 3), Twinnia japonensis (n = 3), and Cnephia mutata (n = 3). Individually extracted DNA from the black flies was subjected to polymerase chain reaction (PCR) amplification targeting the partial mitochondrial cytochrome b gene of birds or mammals to identify the blood meals. Of 144 black flies examined, 34 specimens were PCR positive for avian hosts (23.6%). No mammalian-derived bloods were detected from the samples studied through. Sequences amplified from 11 black flies consist of S. japonicum, P. hirtipes, and C. mutata showed high similarity to that of the Japanese rock ptarmigan. Therefore, present results conclusively suggest that these three species of black flies might suck the bloods of Japanese rock ptarmigans and could be the vector for L. lovati infection among this endangered bird species of Japan.