DETOXIFICATION OF XENOBIOTICS BY GLUTATHIONE S-TRANSFERASES IN ERYTHROCYTES - THE TRANSPORT OF THE CONJUGATE OF GLUTATHIONE AND 1-CHLORO-2,4-DINITROBENZENE
DETOXIFICATION OF XENOBIOTICS BY GLUTATHIONE S-TRANSFERASES IN ERYTHROCYTES - THE TRANSPORT OF THE CONJUGATE OF GLUTATHIONE AND 1-CHLORO-2,4-DINITROBENZENE
复制标题
DOI：
10.1111/j.1365-2141.1983.tb02156.x
复制
发表时间：
1983-01-01
影响因子：
6.5
通讯作者：
SRIVASTAVA, SK
中科院分区：
文献类型：
--
作者：
AWASTHI, YC;MISRA, G;SRIVASTAVA, SK
人红细胞与1-氯-2，4-二硝基苯(CDNB)孵育后，谷胱甘肽(GSH)几乎定量结合生成S-(2，4-二硝基苯基)谷胱甘肽。该反应是由红细胞谷胱甘肽S转移酶催化的。在CDNB处理的红细胞孵育液中检测到结合物，表明GSH和CDNB的结合物是由红细胞转运出去的。用氨基酸分析和薄层层析法对培养上清液中的结合物进行定量，结果表明红细胞转运结合物的速率约为140nmol/h/ml红细胞。NaF抑制了偶联物的转运。从红细胞中耗尽三磷酸腺苷会导致转运速率的显着降低，通过将三磷酸腺苷与腺嘌呤和肌苷孵育，三磷酸腺苷的再生可以使转运速率恢复。共轭化合物的输运显然是一个能量依赖的过程。
The incubation of human erythrocytes with 1-chloro-2,4-dinitrobenzene (CDNB) results in almost quantitative conjugation of glutathione (GSH) to form S-(2,4-dinitrophenyl) glutathione. The reaction is catalyzed by erythrocyte glutathione S-transferase. The conjugate in the incubation medium of CDNB-treated erythrocytes was identified, indicating that the conjugate of GSH and CDNB is transported out by the erythrocytes. Quantitation of the conjugate in the incubation medium by amino acid analysis and TLC indicates that the erythrocytes transport the conjugate at an approximate rate of 140 nmol/h/ml erythrocytes. The transport of the conjugate is inhibited by NaF. Exhaustion of ATP from the erythrocytes results in a significant decrease in the rate of transport which is restored with the regeneration of ATP by incubating the erythrocytes with adenine and inosine. The transport of conjugate apparently is an energy dependent process.