REACTIVITY OF LYMPHOCYTE SUBPOPULATIONS IN HUMAN MIXED LYMPHOCYTE CULTURE
REACTIVITY OF LYMPHOCYTE SUBPOPULATIONS IN HUMAN MIXED LYMPHOCYTE CULTURE
复制标题
人类混合淋巴细胞培养物中淋巴细胞亚群的反应性
DOI：
10.1111/j.1744-313x.1975.tb00499.x
复制
发表时间：
1975
影响因子：
2.2
通讯作者：
M. Takasugi
中科院分区：
文献类型：
--
作者：
G. Opelz;M. Kiuchi;M. Takasugi
在22°C下预培养7-10天的淋巴细胞的混合淋巴细胞培养（MLC）反应中抗原性丧失伴随着骨髓来源的淋巴细胞（B细胞）从22 ± 1%减少至13 ± 1%，胸腺来源的淋巴细胞（T细胞）从65 ± 2%增加至83 ± 1%（P < 0.001）。通过抗人免疫球蛋白包被柱分离或绵羊红细胞（SRBC）玫瑰花结形成从新鲜淋巴细胞悬液中去除B细胞，导致MLC中细胞刺激能力显著降低（P < 0.001）。用兔抗人脑血清包被淋巴细胞可使其在MLC中的反应能力消失，但刺激能力不变。
Loss of antigenicity in the mixed lymphocyte culture (MLC) reaction of lymphocytes precultured at 22°C for 7–10 days was accompanied by a decrease in bone‐marrow‐derived lymphocytes (B cells) from 22 ± 1% to 13 ± 1%, and an increase in thymus‐derived lymphocytes (T cells) from 65 ± 2% to 83 ± 1% (P < 0.001). Depletion of B cells from a fresh lymphocyte suspension by either antihuman immunoglobulin‐coated column fractionation or by sheep red blood cell (SRBC) rosette formation resulted in a significant reduction of the cell's ability to stimulate in MLC (P < 0.001). Coating of lymphocytes with rabbit antihuman brain serum abrogated their ability to respond but not the ability to stimulate in MLC.