Incidence of class 1 integrons in a quaternary ammonium compound-polluted environment
Incidence of class 1 integrons in a quaternary ammonium compound-polluted environment
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DOI：
10.1128/aac.49.5.1802-1807.2005
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发表时间：
2005-05-01
影响因子：
4.9
通讯作者：
Wellington, EMH
中科院分区：
文献类型：
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作者：
Gaze, WH;Abdouslam, N;Wellington, EMH
从一家高使用季铵化合物(QAC)的毛纺精整厂废水治理系统中采集了废水和土壤样品，并与农业土壤样品进行了比较。将革兰氏阴性菌和革兰氏阳性菌分别接种于含5mU g/mlDTDMAC和50mU g/mlDTDMAC或CTAB的营养琼脂上，测定其对二羟甲基氯化铵(DTDMAC)和十六烷基三甲基溴化铵(CTAB)的耐药商。获得了大约500个分离株，并对其进行了intI1(1类整合酶)、qacE(多药排出)和qacE Delta 1(减毒qacE)基因的筛选。芦苇地分离株对QAC的耐药性较高，预先暴露于QAC的人群中1类整合子的发生率显著较高。这是第一个证明在自然环境中QAC选择有可能共选抗生素耐药性的研究，因为1类整合子是编码抗生素耐药性的盒基因的成熟载体。
Samples of effluent and soil were collected from a reed bed system used to remediate liquid waste from a wool finishing mill with a high use of quaternary ammonium compounds (QACs) and were compared with samples of agricultural soils. Resistance quotients of aerobic gram-negative and gram-positive bacteria to ditallowdimethylammomium chloride (DTDMAC) and cetyltrimethylammonium bromide (CTAB) were established by plating onto nutrient agar containing 5 mu g/ml or 50 mu g/ml DTDMAC or CTAB. Approximately 500 isolates were obtained and screened for the presence of the intI1 (class 1 integrase), qacE (multidrug efflux), and qacE Delta 1 (attenuated qacE) genes. QAC resistance was higher in isolates from reed bed samples, and class 1 integron incidence was significantly higher for populations that were preexposed to QACs. This is the first study to demonstrate that QAC selection in the natural environment has the potential to coselect for antibiotic resistance, as class 1 integrons are well-established vectors for cassette genes encoding antibiotic resistance.