Differentiation Induction of Mouse Neural Stem Cells in Hydrogel Tubular Microenvironments with Controlled Tube Dimensions
Differentiation Induction of Mouse Neural Stem Cells in Hydrogel Tubular Microenvironments with Controlled Tube Dimensions
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DOI：
10.1002/adhm.201500903
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发表时间：
2016-05-11
影响因子：
10
通讯作者：
Takeuchi, Shoji
中科院分区：
文献类型：
--
作者：
Onoe, Hiroaki;Kato-Negishi, Midori;Takeuchi, Shoji
在本文中，一个管状的三维微环境中创建的藻酸钙水凝胶微管相对于支架尺寸对小鼠神经干细胞（mNSCs）的分化的影响进行了评估。本发明利用双同轴微流控装置制备了五种不同芯径（约65-200 μ m）和壳厚（约30-110 μ m）的水凝胶微管，通过将生长培养基改为分化培养基诱导包封的mNSCs分化。通过使用定量实时聚合酶链反应和荧光免疫细胞化学的微管几何形状的影响进行检查。分析表明，微管厚度在30-110 μ m的差异影响相对Tuj 1的表达，但不影响封装的mNSCs的形态。核的直径影响Tuj 1的相对表达和分化的神经元的形态。发现具有小于约100 μ m的核心直径的管状微环境有助于形成高度可行和对齐的神经组织。管状微环境可以为构建具有几何控制分化诱导的微纤维状神经组织提供有效的方法。
In this paper, a tubular 3D microenvironment created in a calcium alginate hydrogel microtube with respect to the effect of scaffold dimensions on the differentiation of mouse neuronal stem cells (mNSCs) is evaluated. Five types of hydrogel microtubes with different core diameters (approximate to 65-200 mu m) and shell thicknesses (approximate to 30-110 mu m) are fabricated by using a double coaxial microfluidic device, and differentiation of encapsulated mNSCs is induced by changing the growth medium to the differentiation medium. The influence of the microtube geometries is examined by using quantitative real-time polymerase chain reaction and fluorescent immunocytochemistry. The analyses reveal that differences in microtube thickness within 30-110 mu m affected the relative Tuj1 expression but do not affect the morphology of encapsulated mNSCs. The diameters of cores influence both the relative Tuj1 expression and morphology of the differentiated neurons. It is found that the tubular microenvironment with a core diameter of less than approximate to 100 mu m contributes to forming highly viable and aligned neural tissue. The tubular microenvironment can provide an effective method for constructing microfiber-shaped neural tissues with geometrically controlled differentiation induction.