High-throughput yeast two-hybrid assays for large-scale protein interaction manning
High-throughput yeast two-hybrid assays for large-scale protein interaction manning
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DOI：
10.1006/meth.2001.1190
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发表时间：
2001-07-01
期刊：
影响因子：
4.8
通讯作者：
Vidal, M
中科院分区：
文献类型：
--
作者：
Walhout, AJM;Vidal, M
蛋白质-蛋白质相互作用在许多生物过程中发挥着重要作用。因此，蛋白质相互作用图谱正在成为一种成熟的功能基因组学方法，用于为迄今为止尚未表征的预测蛋白质生成功能注释。酵母双杂交系统是目前最标准化的蛋白质相互作用图谱技术之一。在这里，我们描述了半自动化、高通量、基于 Gal4 的酵母双杂交系统的协议。 (C) 2001 年学术出版社。
Protein-protein interactions play fundamental roles in many biological processes. Hence, protein interaction mapping is becoming a well-established functional genomics approach to generate functional annotations for predicted proteins that so far have remained uncharacterized. The yeast two-hybrid system is currently one of the most standardized protein interaction mapping techniques. Here, we describe the protocols for a semiautomated, high-throughput, Gal4-based yeast two-hybrid system. (C) 2001 Academic Press.