Determination of the Amount of Carbohydrates Adsorbed on Solid Substrates
Determination of the Amount of Carbohydrates Adsorbed on Solid Substrates
复制标题
DOI：
10.1002/pssa.201700845
复制
发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Pariyanka Arenja;C. Rösch;Katharina Huttenlochner;C. Müller‐Renno;M. Hannig;C. Ziegler
中科院分区：
文献类型：
--
作者：
Pariyanka Arenja;C. Rösch;Katharina Huttenlochner;C. Müller‐Renno;M. Hannig;C. Ziegler
碳水化合物，例如以多糖如葡聚糖的形式，是所有活生物体的必需组分，并且参与细胞和细菌粘附。后者可导致生物膜，其可引起疾病或在生物膜反应器中有益。为了利用结合的碳水化合物的效果，需要知道它们在什么条件下结合到固体表面，如牙科材料或生物膜反应器壁。为此，一个有趣的问题是在预定条件下多糖结合到表面的量。然而，结合碳水化合物的定量是非常困难的。用于定量溶液中的碳水化合物的众所周知的方法是苯酚-硫酸（PSA）测定。在此，该方法适用于结合碳水化合物。这种改良的PSA测定法提供了可重复的结果，并且只需要少量的试剂和碳水化合物。在葡聚糖结合在钛上作为生物材料的情况下，它提供了预期的结果，即更高的浓度导致更高的吸附量。
Carbohydrates, for example in form of polysaccharides such as dextran are essential components of all living organisms and are involved in cell and bacterial adhesion. The latter can lead to biofilms which may either cause diseases or are beneficial in biofilm reactors. To utilize effects of bound carbohydrates it is required to know under which conditions they bind to solid surfaces such as dental materials or biofilm reactor walls. For this purpose one interesting question is to which amount the polysaccharides bind to the surface under a predefined condition. However, the quantification of bound carbohydrates is very difficult. A well‐known method to quantify carbohydrates in solution is the phenol‐sulfuric acid (PSA) assay. Here, this method is adapted for bound carbohydrates. This modified PSA assay delivers reproducible results and does only need a small amount of reagents and carbohydrates. In the case of dextran bound on titanium as biomaterial it delivers the expected results, that is that higher concentrations lead to a higher adsorbed amount.