The ethyl acetate fraction of corn silk exhibits dual antioxidant and anti-glycation activities and protects insulin-secreting cells from glucotoxicity.
The ethyl acetate fraction of corn silk exhibits dual antioxidant and anti-glycation activities and protects insulin-secreting cells from glucotoxicity.
复制标题
DOI：
10.1186/s12906-016-1382-8
复制
发表时间：
2016-11-03
影响因子：
--
通讯作者：
Liu HK
中科院分区：
文献类型：
--
作者：
Chang CC;Yuan W;Roan HY;Chang JL;Huang HC;Lee YC;Tsay HJ;Liu HK
在这项研究中，我们的目标是开发一种具有抗氧化和蛋白质糖基化双重生物活性的玉米丝提取物，以保护β细胞免受糖尿病诱导的衰竭。通过分配法制备了玉米丝级分，并用薄层层析法对其进行了化学表征。通过自由基清除试验、糖基化试验和细胞活性试验(中性红)确定最佳组分。膜联蛋白V/碘化丙啶染色进行细胞死亡分析。用WST-1法检测细胞增殖。用β细胞标志物基因表达(RTPCR法)和急性胰岛素分泌试验评价β细胞功能。以玉米丝的乙醇粗提物为原料，制备了四种玉米丝级分。体外试验表明，乙酸乙酯部位(YMS-EA)是最有效的部位。YMS-EA还可减弱过氧化氢或甲基乙二醛诱导的活性氧诱导、细胞活力降低和细胞增殖抑制。然而，YMS-EA不能阻止过氧化氢诱导的细胞凋亡或晚期糖基化终末产物诱导的毒性。在高血糖条件下，YMS-EA可有效降低ROS水平，提高胰岛素、葡萄糖激酶和PDX-1的mRNA表达，并增强葡萄糖刺激的胰岛素分泌。芹菜素、木犀草素和YMS-EA的生物活性相似，表明YMS-EA的双重活性可能来源于这些化合物。结论：玉米素-EA组分可作为预防2型糖尿病β细胞糖毒性的食品制剂。
In this study, we aimed to develop a Stigmata Maydis (corn silk) fraction with dual bio-activities against oxidative stress and protein glycation to protect β-cells from diabetes-induced failure. Corn silk fractions were prepared by partition and chemically characterised by thin-layer chromatography. Free radical scavenging assay, glycation assay, and cell-based viability test (neutral red) were employed to decide the best fraction. Cell death analysis was executed by annexin V/ Propidium iodide staining. Cell proliferation was measured by WST-1. Finally, β-cell function was evaluated by β-cell marker gene expression (RT-PCR) and acute insulin secretion test. Four corn silk fractions were prepared from an ethanolic crude extract of corn silk. In vitro assays indicate ethyl acetate fraction (YMS-EA) was the most potent fraction. YMS-EA also attenuated the hydrogen peroxide- or methylglyoxal-induced induction of reactive oxygen species, reduction of cell viability, and inhibition of cell proliferation. However, YMS-EA was unable to prevent hydrogen peroxide-induced apoptosis or advanced glycation end-products-induced toxicity. Under hyperglycemic conditions, YMS-EA effectively reduced ROS levels, improved mRNA expression of insulin, glucokinase, and PDX-1, and enhanced glucose-stimulated insulin secretion. The similarity of bioactivities among apigenin, luteolin, and YMS-EA indicated that dual activities of YMS-EA might be derived from those compounds. We concluded that YMS-EA fraction could be developed as a preventive food agent against the glucotoxicity to β-cells in Type 2 diabetes.