Comparative Complementation Mapping of Methylophilus spp. Using Cosmid Gene Libraries and Prime Plasmids
Comparative Complementation Mapping of Methylophilus spp. Using Cosmid Gene Libraries and Prime Plasmids
复制标题
嗜甲基菌属的比较互补作图。
DOI：
10.1099/00221287-134-1-123
复制
发表时间：
1988
期刊：
影响因子：
1.5
通讯作者：
B. Holloway
中科院分区：
文献类型：
--
作者：
B. R. Lyon;P. Kearney;M. Sinclair;B. Holloway
总结：通过将染色体DNA的Sau 3A部分片段连接到广宿主范围粘粒载体pLA 2917中，产生专性甲基营养菌Methylophilus viscogenes和Methylophilus methylotrophus AS 1的粘粒基因文库。遗传连锁群被确定为每种生物体的重组cosestrin动员后，以广泛的铜绿假单胞菌和大肠杆菌营养缺陷型的互补。由此获得的连锁数据证实和扩展了那些先前建立的总理质粒互补，和两个甲基营养型显示非常相似的安排研究的四个基因簇。然而，互补相同营养缺陷型标记的DNA序列的比较未能证明两种甲基营养型之间的限制性片段同一性或实质性DNA-DNA同源性。
SUMMARY: Cosmid gene libraries of the obligate methylotrophs Methylophilus viscogenes and Methylophilus methylotrophus AS1 were generated by ligating Sau3A partial digests of chromosomal DNA into the broad-host-range cosmid vector pLA2917. Genetic linkage groups were identified for each organism by complementation following mobilization of the recombinant cosmids to a wide range of Pseudomonas aeruginosa and Escherichia coli auxotrophs. The linkage data thus obtained confirmed and extended those previously established by prime plasmid complementation, and the two methylotrophs showed very similar arrangements for four of the gene clusters studied. However, comparison of DNA sequences which complemented the same auxotrophic markers failed to demonstrate restriction fragment identity or substantial DNA-DNA homology between the two methylotrophs.