Evolutionary dynamics of fitness recovery from the debilitating effects of Muller's ratchet
Evolutionary dynamics of fitness recovery from the debilitating effects of Muller's ratchet
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DOI：
10.1111/j.1558-5646.1998.tb01633.x
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发表时间：
1998-04-01
期刊：
影响因子：
3.3
通讯作者：
Moya, A
中科院分区：
文献类型：
--
作者：
Elena, SF;D치vila, M;Moya, A
RNA病毒表现出的强大适应性是其高突变率的结果。在五种不同的人口统计制度下，比较了严重衰弱的非节段核糖病毒水泡性口炎病毒（VSV）克隆群体的适应度进化，范围从严重的连续瓶颈传代（病毒粒子）到相似环境条件（细胞培养类型和温度）下的大群体传代（10（6）个病毒粒子或更多）。无论瓶颈有多小，进化种群的适应度总是高于起始种群的适应度；这一结果明显不同于之前报道的具有更高适应度的病毒的结果。在连续群体传代的情况下，适应性的重新获得表现出两个主要特征：（1）早期传代的适应率较高； (2)经过大量传代后达到最大适应度值。这种最初衰弱的克隆达到的最大适应度与野生型病毒的适应度相似。还讨论了这些发现对使用减毒病毒设计疫苗的实际影响。
The great adaptability shown by RNA viruses is a consequence of their high mutation rates. The evolution of fitness in a severely debilitated, clonal population of the nonsegmented ribovirus vesicular stomatitis virus (VSV) has been compared under five different demographic regimes, ranging from severe serial bottleneck passages tone virion) to large population passages (10(6) virions or more) under similar environmental conditions (cell culture type and temperature). No matter how small the bottleneck, the fitness of the evolved populations was always higher than the fitness of the starting population; this result is clearly different from that previously reported for viruses with higher fitness. The reattainment of fitness under a regime of serial population passages showed two main characteristics: (1) the rate of adaptation was higher during early passages; and (2) a maximum fitness value was reached after a large number of passages. The maximum fitness reached by this initially debilitated clone was similar to the fitness of wild-type virus. The practical implications of these findings in the design of vaccines using attenuated viruses are also discussed.