Odontoblast-like cell numbers and reparative dentine thickness after direct pulp capping with platelet-rich plasma and enamel matrix derivative: a histomorphometric evaluation
Odontoblast-like cell numbers and reparative dentine thickness after direct pulp capping with platelet-rich plasma and enamel matrix derivative: a histomorphometric evaluation
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DOI：
10.1111/j.1365-2591.2011.01977.x
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发表时间：
2012-04-01
影响因子：
5
通讯作者：
Senguuven, B.
中科院分区：
文献类型：
--
作者：
Orhan, E. O.;Maden, M.;Senguuven, B.
目的 收集富血小板血浆（PRP）和牙釉质基质衍生物（EMD）直接盖髓后成牙本质细胞样细胞数量和修复牙本质厚度的定量信息。方法 该实验在 36 只 Wistar 白化大鼠和总共 144 颗门牙上进行。将氢氧化钙、三氧化二矿物质骨料、PRP 和 EMD 作为直接封盖剂应用于 96 颗门牙 (n = 24) 的牙髓。在其余 48 颗门牙上创建阳性和阴性对照组。第7天和第28天拔牙。常规组织学制备后，用苏木精和伊红对横截面进行染色。在第7天和第28天通过组织形态计量学测量成牙本质细胞样细胞的数量。还测量了修复牙本质的厚度。还测量了牙本质桥下方的成牙本质细胞样细胞的数量。所有数据的正态分布均通过 MannWhitney U 检验进行检验。使用 KruskalWallis 检验分析组间的统计差异。结果 与实验组和阴性对照组相比，除 EMD 组外（P < 0.01），牙髓暴露后第 7 天至 28 天，所有组中成牙本质细胞样细胞的平均数量均增加（P > 0.01）（P < 0.01）。在所有牙齿组中均观察到修复性牙本质形成（P > 0.01）。结论 观察到修复性牙本质形成，但组间无显着差异。观察到成牙本质细胞样细胞与用 PRP 和 EMD 覆盖的牙髓的结果相关。 PRP 和 EMD 是可能影响修复性牙本质形成厚度的封盖剂。
Aim To collect quantitative information about the numbers of odontoblast-like cells and reparative dentine thickness after direct pulp capping with platelet-rich plasma (PRP) and enamel matrix derivative (EMD). Methodology The experiment was conducted on 36 Wistar albino rats and a total of 144 incisor teeth. Calcium hydroxide, mineral trioxide aggregate, PRP and EMD were applied as direct capping agents on the pulps of 96 incisors (n = 24). Positive and negative control groups were created on the remaining 48 incisors. The teeth were extracted on the 7th and 28th days. After routine histological preparation, cross-sections were stained with haematoxylin and eosin. The numbers of the odontoblast-like cells were measured histomorphometrically on day 7 and day 28. The thickness of the reparative dentine was also measured. The number of odontoblast-like cells was also measured beneath the dentine bridge. The normal distribution of all data was tested with the MannWhitney U test. The statistical differences between groups were analysed using the KruskalWallis test. Results The mean number of odontoblast-like cells increased between day 7 and 28 following pulp exposure (P > 0.01) in all groups except for the EMD group (P < 0.01), when compared with both the experimental and negative control groups (P < 0.01). Reparative dentine formation was observed in all groups of teeth (P > 0.01). Conclusions Reparative dentine formation was observed, but with no significant difference between the groups. Odontoblast-like cells were observed in association with the outcome of pulps capped with PRP and EMD. PRP and EMD are possible capping agents that influence the thickness of reparative dentine formation.