Quantitative analysis of acrylamide in tea by liquid chromatography coupled with electrospray ionization tandem mass spectrometry.
Quantitative analysis of acrylamide in tea by liquid chromatography coupled with electrospray ionization tandem mass spectrometry.
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DOI：
10.1016/j.foodchem.2007.11.015
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发表时间：
2008-05
期刊：
影响因子：
8.8
通讯作者：
Jie Liu;Gang Zhao;Yuan Yuan-Yuan;Fang Chen;Xiaosong Hu
中科院分区：
文献类型：
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作者：
Jie Liu;Gang Zhao;Yuan Yuan-Yuan;Fang Chen;Xiaosong Hu
优化了有效的样品前处理方法，建立了茶叶中丙烯酰胺的LC-MS/MS定量分析方法。[13C3]-丙烯酰胺为内标。在25℃下用10ml水和10ml乙腈提取丙烯酰胺20min，然后在搅拌下向上述混合物中加入4g硫酸镁和0.5g氯化钠。为了提高丙烯酰胺的响应性，取9ml乙腈层，浓缩至0.5ml。采用OASIS MCX色谱柱进行固相萃取净化。检测限(LOD)为1 ng/ml，定量限(LOQ)为5 ng/ml。提取过程的回收率在74%~79%之间。30个茶叶样品的丙烯酰胺含量均低于100 ng/g，其中红茶、乌龙茶、白茶和黄茶的丙烯酰胺含量较低，为20 ng/g。烘焙、烘焙和晒干绿茶样品中的丙烯酰胺含量较高(46-94 ng/g)。
An effective sample preparation procedure was optimized and a liquid chromatography–tandem mass spectrometry (LC–MS/MS) was developed for the quantitative analysis of acrylamide in tea. [13C3]-acrylamide was used as internal standard. Acrylamide was extracted at 25°C for 20min by 10ml water followed by 10ml acetonitrile, and then 4g of magnesium sulfate and 0.5g of sodium chloride were added to the above mixture under stirring thoroughly. In order to increase the response of acrylamide, 9ml acetonitrile layer was taken and concentrated to 0.5ml. Solid-phase extraction with an Oasis MCX cartridge was carried out for clean-up. The limit of detection (LOD) and limit of quantification (LOQ) were 1 and 5ng/ml, respectively. The recovery efficiency of the extraction procedure ranged between 74% and 79%. The levels of acrylamide in 30 tea samples were less than 100ng/g. Black, oolong, white and yellow tea samples had quite low acrylamide contents (<20ng/g). Higher acrylamide levels occurred in baked, roasted, and one sun-dried green tea samples (46–94ng/g).