Titanium dioxide nanotube films Preparation, characterization and electrochemical biosensitivity towards alkaline phosphatase
Titanium dioxide nanotube films Preparation, characterization and electrochemical biosensitivity towards alkaline phosphatase
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DOI：
10.1016/j.msec.2014.01.036
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发表时间：
2014-04-01
影响因子：
7.9
通讯作者：
Dinischiotu, Anca
中科院分区：
文献类型：
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作者：
Roman, Ioan;Trusca, Roxana Doina;Dinischiotu, Anca
采用乙二醇和甘油在不同基体（钛、Ti6Al4V和Ti6Al7Nb合金）上阳极氧化法制备了钛纳米管。分析了外加电位和处理时间对纳米管直径和长度的影响。形成的纳米管层是无定形的，但当在550℃的空气中进行后续热处理时，它们变成结晶；在钛和Ti6Al4V合金基体上生长的TNT层主要由锐钛矿和金红石组成，而在Ti6Al7Nb合金基体上生长的TNT层主要由锐钛矿组成。在Ti6Al7Nb合金上生长的纳米管层不均匀，在表面上散布着直径较小的纳米管岛。与未经处理的钛板相比，在三种基质上生长的纳米管具有更好的成骨细胞粘附和增殖能力。采用电化学阻抗谱法考察了TNT/Ti样品对牛碱性磷酸酶的敏感性与样品的结晶度、直径和阳极电解液性质的关系。在0.005 ~ 0.1 mg/mL的浓度范围内，对直径为50 nm的退火纳米管在甘油中生长的测量能力进行了验证，建立了相应的校准曲线。(C) 2014 Elsevier B.V.版权所有
Titania nanotubes (TNTs) were prepared by anodization on different substrates (titanium, Ti6Al4V and Ti6Al7Nb alloys) in ethylene glycol and glycerol. The influence of the applied potential and processing time on the nanotube diameter and length is analyzed. The as-formed nanotube layers are amorphous but they become crystalline when subjected to subsequent thermal treatment in air at 550 degrees C; TNT layers grown on titanium and Ti6Al4V alloy substrates consist of anatase and rutile, while those grown on Ti6Al7Nb alloy consist only of anatase. The nanotube layers grown on Ti6Al7Nb alloy are less homogeneous, with supplementary islands of smaller diameter nanotubes, spread across the surface. Better adhesion and proliferation of osteoblasts was found for the nanotubes grown on all three substrates by comparison to an unprocessed titanium plate. The sensitivity towards bovine alkaline phosphatase was investigated mainly by electrochemical impedance spectroscopy in relation to the crystallinity, the diameter and the nature of the anodization electrolyte of the TNT/Ti samples. The measuring capacity of the annealed nanotubes of 50 nm diameter grown in glycerol was demonstrated and the corresponding calibration curve was built for the concentration range of 0.005-0.1 mg/mL. (C) 2014 Elsevier B.V. All rights reserved.