Quantitative proteomics revealed modulation of macrophages by MetQ gene of Streptococcus suis serotype 2.
Quantitative proteomics revealed modulation of macrophages by MetQ gene of Streptococcus suis serotype 2.
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定量蛋白质组学揭示了猪链球菌血清型 2 的 MetQ 基因对巨噬细胞的调节作用。
DOI：
10.1186/s13568-020-01131-2
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发表时间：
2020-10-30
期刊：
影响因子：
3.7
通讯作者：
Fan H
中科院分区：
文献类型：
--
作者：
Pei X;Liu J;Liu M;Zhou H;Wang X;Fan H
猪链球菌2型（Streptococcus suis serotype 2，SS 2）是一种严重的人畜共患病原菌，可引起人链球菌中毒性休克综合征（Streptococcus toxic shock syndrome，STSS）和猪败血症，对公众健康构成极大威胁。SS 2 MetQ基因缺失菌株具有减弱的抗吞噬作用，尽管SS 2中MetQ的抗吞噬作用和发病机制仍不清楚。本研究采用基于液相色谱-质谱（LC-MS）技术的细胞培养中氨基酸稳定同位素标记（SILAC）技术，并结合生物信息学分析方法，对△MetQ和ZY 05719感染RAW 264. 7细胞后差异表达的蛋白质进行分析。通过定量实时聚合酶链反应（qRT-PCR）和蛋白质印迹法对所选蛋白质的蛋白质组学结果进行验证。进一步的研究主要集中在与下调的蛋白质（如Src和Ccl 9）以及肌动蛋白细胞骨架和内吞相关的上调蛋白质（如Pstpip 1和Ppp 1 r9 b）相关的免疫系统过程。本研究的蛋白质组学结果揭示了△MetQ和ZY 05719感染巨噬细胞的抗吞噬和天然免疫机制，为预防或控制SS 2感染提供了新的靶点。
Streptococcus suis serotype 2 (SS2) is a serious zoonotic pathogen; it can lead to symptoms of streptococcal toxic shock syndrome (STSS) in humans and sepsis in pigs, and poses a great threat to public health. The SS2 MetQ gene deletion strain has attenuated antiphagocytosis, although the mechanism of antiphagocytosis and pathogenesis of MetQ in SS2 has remained unclear. In this study, stable isotope labeling by amino acids in cell culture (SILAC) based liquid chromatography–mass spectrometry (LC–MS) and subsequent bioinformatics analysis was used to determine differentially expressed proteins of RAW264.7 cells infected with △MetQ and ZY05719. Proteomic results were verified by quantitative real-time polymerase chain reaction (qRT-PCR) and Western blotting for selected proteins. Further research was focused mainly on immune system processes related to downregulated proteins, such as Src and Ccl9, and actin cytoskeleton and endocytosis related upregulated proteins, like Pstpip1 and Ppp1r9b. The proteomic results in this study shed light on the mechanism of antiphagocytosis and innate immunity of macrophages infected with △MetQ and ZY05719, which might provide novel targets to prevent or control the infection of SS2.
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影响因子：
3.7
作者：
Basavanna S;Chimalapati S;Maqbool A;Rubbo B;Yuste J;Wilson RJ;Hosie A;Ogunniyi AD;Paton JC;Thomas G;Brown JS
通讯作者：
Brown JS
影响因子：
3.1
作者：
Killpack, Tess L.;Ballesteros, Maria;Petnicki-Ocwieja, Tanja
通讯作者：
Petnicki-Ocwieja, Tanja
影响因子：
4.8
作者：
Ong, Seow Theng;Freeley, Michael;Long, Aideen
通讯作者：
Long, Aideen
影响因子：
4.2
作者：
Gu, Hongwei;Zhu, Haodan;Lu, Chengping
通讯作者：
Lu, Chengping
影响因子：
3.1
作者：
Ejim, LJ;D'Costa, VM;Wright, GD
通讯作者：
Wright, GD