Genetic architecture of qualitative and quantitative Melampsora larici-populina leaf rust resistance in hybrid poplar:: genetic mapping and QTL detection
Genetic architecture of qualitative and quantitative Melampsora larici-populina leaf rust resistance in hybrid poplar:: genetic mapping and QTL detection
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DOI：
10.1111/j.1469-8137.2005.01424.x
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发表时间：
2005-07-01
期刊：
影响因子：
9.4
通讯作者：
Bastien, C
中科院分区：
文献类型：
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作者：
Jorge, V;Dowkiw, A;Bastien, C
为了阐明杂交杨叶锈病抗性的遗传控制机制，对一株三角杨（Populus deltoides）与毛卡柏（P. trichocarpa F(1)）后代的叶锈病抗性进行了定性和定量分析。该子代在控制条件下对7株落叶松-populina菌株进行了3个定量抗性成分（潜伏期、尿尿素数量和尿尿素大小）和1个田间敏感性成分（感染最严重的叶片上的锈病定殖）的评估。在遗传图谱上定位了从三角木遗传的一个质抗性位点R(1)。它在F(1)子代中分离为1:1，对所研究的四种菌株有效。分别对R(1)和R(1)基因型亚群进行QTL分析。对能够克服R(1)和MAX2的3株菌株进行了整个F(1)子代的检测。共检测到9个qtl。其中两种具有广谱效应。一个（R(US)）遗传自P. trichocarpa亲本；另一个遗传自P. deltoides并与R(1)共定位。7个qtl只有有限和特定的影响。主要在两个主要qtl之间存在显著的互作效应。讨论了这些结果对持久抗性育种策略的影响，以及杨树基因组序列可能带来的好处。
In order to elucidate the genetic control of resistance to Melampsora larici-populina leaf rust in hybrid poplars, a Populus deltoides x P. trichocarpa F(1) progeny was analysed for qualitative and quantitative rust resistances.This progeny was evaluated for three components of quantitative resistance (latent period, uredinia number and uredinia size) to seven M. larici-populina strains in controlled conditions, and for one component of field susceptibility (rust colonization on the most infected leaf).One qualitative resistance locus inherited from P. deltoides, R(1), was localized on the genetic map. It segregates 1 : 1 in the F(1) progeny and is effective against four of the studied strains. QTL analysis was performed separately on R(1) and r(1) genotype subsets. An additional detection was conducted on the entire F(1) progeny for the three strains able to overcome R(1) and for MAX2. A total of nine QTLs were detected. Two had large, broad-spectrum effects. One (R(US)) is inherited from the P. trichocarpa parent; the other is inherited from P. deltoides and colocalized with R(1). Seven QTLs had only limited and specific effects. Significant interaction effects were detected mainly between the two major QTLs.Implications of these results for durable resistance breeding strategies, and possible benefits from the Populus genome sequence, are discussed.