Optimizing signal and mass resolution for matrix-assisted laser desorption utilizing a linear time-of-flight mass spectrometer.
Optimizing signal and mass resolution for matrix-assisted laser desorption utilizing a linear time-of-flight mass spectrometer.
复制标题
利用线性飞行时间质谱仪优化基质辅助激光解吸的信号和质量分辨率。
DOI：
10.1002/rcm.1290061113
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发表时间：
1992
期刊：
影响因子：
--
通讯作者：
Gilfrich,NL
中科院分区：
文献类型：
--
作者：
Brown,RS;Gilfrich,NL
本文给出了用于矩阵辅助激光解吸质谱分析的各种仪器配置的结果。用线性飞行时间质谱仪对不同长度的无场区进行了质量分辨率的测定。利用导线离子导流器，可以提高较长无场区域的离子传输效率。实验还确定，与没有金属丝离子导轨的相同几何形状的质量分辨率相比，采用金属丝离子导轨的配置通常获得适度的质量分辨率增加。确定了导线离子导向器的最佳应用电位。对于马肌红蛋白（MW 16 951.5 Da）和细菌蛋白酶（MW 27 228.4 Da）样品，观察到导线离子向导的最佳施加电位（- 100 V）没有质量依赖性。兔γ球蛋白（MW≈︁150000 Da）的单电荷和四电荷分子离子的最佳施加电压也相同（- 100 V）。结果表明，带导线离子导管的2 m飞行管比不带导线离子导管的1 m飞行管提供了更好的信噪比质谱，并且可以获得两倍以上的质量分辨率。利用获得。利用4米飞行管，以牺牲信噪比为代价，获得最小的质量分辨率增强。
Results are oresented for various instrumental configurations employed for matrix‐assisted laser desorption mass spectrometry. Mass resolution is determined for a linear time‐of‐flight mass spectrometer for various lengths of the field‐free region. A wire ion guide is utilized and is shown to improve ion transport efficiencies for longer field‐free regions. It is also determined experimentally that a modest mass resolution increase is often obtained in configurations employing the wire ion guide when compared to the mass resolution obtained with the same geometr without the wire ion guide. Optimal applied potentials are determined for the wire ion guide. No mass dependence on the opitmal applied potential (−100 V) for the wire ion guide is observed for samples of equine myoglobin (MW 16 951.5 Da) and a bacterial protease (MW 27 228.4 Da). The optimal applied voltage was also found to b identical (−100 V) for the singly through quadruply charged molecular ion species of rabbit gamma globulin (MW ≈︁ 150 000 Da). It is shown that a 2 m flight tube with a wire ion guide provides better signal‐to‐noise mass spectra than a 1 m flight tube without the wire ion guide and can more than double the mass resolution obtainable. Utilization obtainable. Utilization of a 4 m flight tube gives minimal mass resolution enhancement at the expense of signal‐to‐noise.