The characterization of photoinhibition and recovery during cold acclimation in Arabidopsis thaliana using chlorophyll fluorescence imaging
The characterization of photoinhibition and recovery during cold acclimation in Arabidopsis thaliana using chlorophyll fluorescence imaging
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DOI：
10.1034/j.1399-3054.2003.00224.x
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发表时间：
2003-11-01
影响因子：
6.4
通讯作者：
Whitehead, CL
中科院分区：
文献类型：
--
作者：
Gray, GR;Hope, BJ;Whitehead, CL
研究了拟南芥（Columbia生态型）在4℃冷驯化条件下、23℃非驯化条件下和4℃冷驯化条件下对光合作用的光抑制及其恢复的响应。这些反应是用Chl荧光成像测定的。我们表明，与未驯化的植物相比，冷驯化导致对光抑制的耐受性增加，并且低温下的生长和发育对这种情况的发生至关重要。冷移植物的耐受性不如冷驯化植物。此外，我们证明了这种耐受性是在高PSII激发压力下生长的结果，可以通过生长温度或生长辐照度来调节。冷驯化和冷移植物在黑暗中完全从光抑制中恢复，而未驯化的植物显示出较低的恢复水平，并表现出对光的需求。讨论了PSII修复周期的作用，PSII猝灭中心，以及利用Chl荧光成像监测植物的光抑制反应。
The responses to photoinhibition of photosynthesis at low temperature and subsequent recovery were examined in Arabidopsis thaliana (ecotype Columbia) developed at 4degreesC cold-acclimating conditions, 23degreesC non-acclimating conditions and for non-acclimated plants shifted to 4degreesC (cold-shifted). These responses were determined in planta using Chl fluorescence imaging. We show that cold acclimation results in an increased tolerance to photoinhibition in comparison with non-acclimated plants and that growth and development at low temperature is essential for this to occur. Cold-shifted plants were not as tolerant as the cold-acclimated plants. In addition, we demonstrate this tolerance is as a result of growth under high PSII excitation pressure, that can be modulated by growth temperature or growth irradiance. Cold-acclimated and cold-shifted plants fully recover from photoinhibition in the dark, whereas non-acclimated plants show reduced levels of recovery and demonstrate a requirement for light. The role of the PSII repair cycle, PSII quenching centres, and the use of Chl fluorescence imaging to monitor photoinhibitory responses in planta are discussed.