Arfs, phosphoinositides and membrane traffic
Arfs, phosphoinositides and membrane traffic
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DOI：
10.1042/bst0331276
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发表时间：
2005-12-01
影响因子：
3.9
通讯作者：
Donaldson, JG
中科院分区：
文献类型：
--
作者：
Donaldson, JG
Arf（ADP-核糖基化因子）GTP结合蛋白在细胞中起调节膜运输和结构的作用。Arfs通过修饰膜脂质和将蛋白质（包括外壳蛋白和肌动蛋白）募集到膜表面来完成这一任务。Arf 1和Arf 6是最不同和研究最多的人类Arf蛋白，其分别主要定位于高尔基复合体和质膜。我们一直在研究Arf 1和Arf 6对这些特定隔室的靶向作用，以及它们对这些膜施加的共同和不同的活性。我们已经发现，Arf 6通过激活I型磷脂酰肌醇4-磷酸S-激酶产生磷脂酰肌醇4，5-二磷酸，并且这种活性有助于促进肌动蛋白细胞骨架重排和观察到的Arf 6激活增加的内体膜运输的改变。Arf 1还可以刺激磷脂酰肌醇激酶的活性，并将外壳蛋白和肌动蛋白细胞骨架元件募集到高尔基复合体。
Arf (ADP-ribosylation factor) GTP-binding proteins function in cells to regulate membrane traffic and structure. Arfs accomplish this task through modification of membrane lipids and the recruitment of proteins, including coat proteins and actin, to membrane surfaces. Arf1 and Arf6 are the most divergent and most studied human Arf proteins that localize predominantly to the Golgi complex and plasma membrane respectively. We have been studying the targeting of Arf1 and Arf6 to these specific compartments and the common and divergent activities that they exert on these membranes. We have found that Arf6 acts through activation of type I phosphaticlylincisitol 4-phosphate S-kinases to generate phosphaticlylinositol 4,5-bisphosphate and that this activity is instrumental in facilitating the actin cytoskeletal rearrangements and alterations in endosomal membrane trafficking observed with increased Arf6 activation. Arf1 can also stimulate the activity of phosphatidylinositol kinases and recruit coat proteins and actin cytoskeletal elements to the Golgi complex.