Elevated Serum Levels of Soluble CD163 in Polymyositis and Dermatomyositis: Associated with Macrophage Infiltration in Muscle Tissue
Elevated Serum Levels of Soluble CD163 in Polymyositis and Dermatomyositis: Associated with Macrophage Infiltration in Muscle Tissue
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DOI：
10.3899/jrheum.141307
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发表时间：
2015-06-01
影响因子：
3.9
通讯作者：
Wang, Guo-Chun
中科院分区：
文献类型：
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作者：
Peng, Qing-Lin;Zhang, Yin-Li;Wang, Guo-Chun
客观的。调查多发性肌炎 (PM) 和皮肌炎 (DM) 患者的可溶性 CD163 (sCD163) 血清水平，并将其与临床表现和实验室数据相关联。方法。采用ELISA法检测24例PM患者、84例DM患者和46例健康对照者血清中sCD163的水平。使用抗 CD163 单克隆抗体对 13 名 PM 患者和 17 名 DM 患者的肌肉活检标本进行肌肉组织中巨噬细胞浸润的免疫组织化学染色。结果。与健康对照相比，患者的 sCD163 血清水平显着升高 (p < 0.001)。患有间质性肺疾病 (ILD) 的患者的 sCD163 水平在统计学上高于无 ILD 的患者 (p < 0.001)。高血清 sCD163 水平与抗核抗体发生率增加 (p < 0.05)、血清免疫球蛋白 G (p < 0.01) 和免疫球蛋白 A (p < 0.05) 水平升高以及红细胞沉降率增加 (p < 0.01) 相关。血清 sCD163 水平与患者外周血 CD3+ T 细胞计数呈负相关（r = -0.306，p < 0.01）。横断面评估和纵向研究揭示了血清 sCD163 水平与疾病活动度之间的显着相关性。血清sCD163水平高的患者肌肉组织中CD163+巨噬细胞浸润的发生率高于sCD163水平正常的患者（卡方值= 10.804，p < 0.01）。结论。 PM/DM 患者的血清 sCD163 水平显着升高，并且与疾病严重程度相关，表明血清 sCD163 作为 PM/DM 疾病评估中有希望的生物标志物。我们发现血清 sCD163 水平升高与肌肉巨噬细胞浸润相关，这凸显了活化的巨噬细胞在 PM/DM 发病机制中的作用。
Objective. To investigate serum levels of soluble CD163 (sCD163) in patients with polymyositis (PM) and dermatomyositis (DM), and to correlate these to clinical manifestations and laboratory data.Methods. Serum levels of sCD163 were detected in 24 patients with PM, 84 patients with DM, and 46 healthy controls by using the ELISA method. Immunohistochemistry staining of macrophage infiltration in muscle tissue using anti-CD163 monoclonal antibody was conducted on muscle biopsy specimens from 13 patients with PM and 17 with DM.Results. Serum levels of sCD163 were significantly increased in patients compared with healthy controls (p < 0.001). Patients with interstitial lung disease (ILD) had statistically higher sCD163 levels than patients without ILD (p < 0.001). High serum sCD163 levels were associated with increased incidence of antinuclear antibody (p < 0.05), higher serum levels of immunoglobulin G (p < 0.01) and immunoglobulin A (p < 0.05), and increased erythrocyte sedimentation rates (p < 0.01). Serum sCD163 levels were inversely correlated with CD3+ T cell counts in peripheral blood of patients (r = -0.306, p < 0.01). Cross-sectional assessment and longitudinal study revealed a significant correlation between serum sCD163 levels and disease activity. Patients with high serum sCD163 levels showed a higher incidence of CD163+ macrophage infiltration in muscle tissue than patients with normal sCD163 levels (chi-square value = 10.804, p < 0.01).Conclusion. Serum levels of sCD163 were significantly elevated and correlated with disease severity in patients with PM/DM, suggesting serum sCD163 as a promising biomarker in the disease evaluation of PM/DM. Our finding of elevated serum sCD163 levels associated with muscle macrophage infiltration highlights the role activated macrophage plays in the pathogenesis of PM/DM.