A homolog of male sex-determining factor SRY cooperates with a transposon-derived CENP-B protein to control sex-specific directed recombination.
A homolog of male sex-determining factor SRY cooperates with a transposon-derived CENP-B protein to control sex-specific directed recombination.
复制标题
男性性别决定因子 SRY 的同源物与转座子衍生的 CENP-B 蛋白合作控制性别特异性定向重组。
DOI：
10.1073/pnas.1109988108
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发表时间：
2011
影响因子：
11.1
通讯作者：
Grewal,ShivIS
中科院分区：
文献类型：
--
作者：
Matsuda,Emiko;Sugioka-Sugiyama,Rie;Mizuguchi,Takeshi;Mehta,Sameet;Cui,Bowen;Grewal,ShivIS
Schizosaccharomyces pombecells switch mating type by replacing genetic information at the expressedmat1locus with sequences copied frommat2-Pormat3-Msilent donor loci. The choice of donor locus is dictated by cell type, such thatmat2is the preferred donor inMcells andmat3is the preferred donor inPcells. Donor choice involves a recombination-promoting complex (RPC) containing Swi2 and Swi5. InPcells, the RPC localizes to a specific DNA element located adjacent tomat3, but inMcells it spreads across the silent mating-type region, includingmat2-P. This differential distribution of the RPC regulates nonrandom choice of donors. However, cell-type–specific differences in RPC localization are not understood. Here we show that themat1-M–encoded factor Mc, which shares structural and functional similarities with the male sex-determining factor SRY, is highly enriched at theswi2andswi5loci and promotes elevated levels of RPC components. Loss of Mc reduces Swi2 and Swi5 to levels comparable to those inPcells and disrupts RPC spreading across themat2/3region. Mc also localizes to loci expressed preferentially inMcells and to retrotransposon LTRs. We demonstrate that Mc localization at LTRs and atswi2requires Abp1, a homolog of transposon-derived CENP-B protein and that loss of Abp1 impairs Swi2 protein expression and the donor choice mechanism. These results suggest that Mc modulates levels of recombination factors, which is important for mating-type donor selection and for the biased gene conversion observed during meiosis, whereMcells serve as preferential donors of genetic information.
